ReliaPrep™ Large Volume HT gDNA Isolation System

Scalable, Walkaway DNA Purification from Large-Volume Blood Samples

The ReliaPrep™ Large Volume HT gDNA Isolation System is used with the HSM 2.0 Instrument (Cat.# A2715) to provide a scalable, efficient method for isolating genomic DNA (gDNA) from 1–10ml blood samples. The precipitation-free ReliaPrep™ DNA purification chemistry eliminates the tedious centrifugation steps and hazardous chemicals associated with precipitation-based methods, reducing purification failures. Each kit provides enough reagents to process up to 96 × 10ml whole blood samples.

The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of 1–10ml whole blood, regardless of sample storage or shipping conditions.

Genomic DNA yields from 10ml normal whole blood samples are typically 200–400µg (depending on white blood cell count) in a final eluted volume of 1ml. Recovered DNA exhibits good purity with A₂₆₀/A₂₈₀ ratios greater than 1.7 and A₂₆₀/A₂₃₀ ratios between 1.8 and 2.2.

Products may not be available in all countries.

Download our Green Sheet to learn more about the features that make this an Environmentally Preferable Product:

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Expedite Sample Processing with the HSM 2.0 Heater Shaker Magnet

The HSM 2.0 Instrument heats, shakes and magnetizes sample tubes in one position throughout the extraction process from lysis to elution, eliminating the need to move samples on the robot deck and reducing instrument failures.

For low-throughput isolation of gDNA from up to 32 samples at one time, the HSM 2.0 instrument can be used in a semi-automated mode, where the user performs the pipetting functions. The HSM has software that controls the instrument and directs the user through the purification protocol.

No Manual Sample Transfer Once Instrument Is Loaded

Simply place the primary blood sample tubes on the instrument or transfer samples to processing tubes, follow the setup instructions and start the protocol. Purified DNA is ready in ≤4.5 hours.

Automated DNA purification from Blood, reliaprep method 8525MB-W

Process Multiple Sample Types

See the Application Notes linked below for details on purification of DNA from blood, buffy coat and packed cell pellet samples using the ReliaPrep™ Large Volume HT gDNA Isolation System.

Sample Type	Promega	External	Comments/Reference
Human Whole Blood	Yes	No	Isolating gDNA from Blood: Simplified gDNA Isolation from Human Blood using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN186, Promega Corporation
Buffy Coat	Yes	No	Isolating gDNA from Buffy Coat Samples: Semi-Automated or Fully Automated Isolation of gDNA from Buffy Coat Samples Using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN185, Promega Corporation

Reagents Available Separately

Product	Size	Cat.#
RNase A Solution	4mg/ml	A7974
20X TE Buffer (pH 7.5)	25ml	A2651
Tissue Lysis Buffer (TLA)	500ml	A5091
Nuclease-Free Water	1,000ml	P1199
Integrated Reagent Caps	4/pk	A2701

HSM 2.0 Ordering Information

Product	Cat.#

Service Agreements

Product	Cat.#

Protocolos

Complete Protocol

ReliaPrep™ Large Volume HT gDNA Isolation System Technical ManualPDF (623 KB) – English

Protocolos en Vídeo

Contenido

Item	Part #	Presentación	Available Separately
Alkaline Protease (APA)	A1721	1 × 130ml	View Product
Cell Lysis Buffer (CLD)	A1731	1 × 1,400ml	View Product
Binding Buffer (BBA)	A1741	1 × 1,600ml	View Product
ReliaPrep™ Resin	A1752	1 × 115ml	View Product
Prepared Wash Buffer (WBC)	A2681	1 × 3,500ml	View Product
Proteinase K (PK) Solution	A5051	1 × 23ml	View Product
Nuclease-Free Water	P1195	4 × 150ml	View Product

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. No. 6,855,499 and other patents.

Recursos

Artículos

Menciones

Challenges in Next Generation Sequencing Analysis of Somatic Mutations in Transplant Patients

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2018 Cancer Genetics
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ReliaPrep™ Large Volume HT gDNA Isolation System

Automated DNA Isolation from 1–10ml of Blood

Scalable, Walkaway DNA Purification from Large-Volume Blood Samples

Expedite Sample Processing with the HSM 2.0 Heater Shaker Magnet

No Manual Sample Transfer Once Instrument Is Loaded

Process Multiple Sample Types