Maxwell® 16 LEV Plant RNA Kit

Faster RNA Purification, Less Hands-On Time and More Efficient Workflows

The Maxwell® 16 LEV Plant RNA Kit is used with the Maxwell® 16 Research Instrument (Cat.# AS2000) to provide an easy method for efficient, automated purification of RNA from plant tissue samples. The Maxwell® 16 Instrument is supplied with preprogrammed purification methods and is designed for use with the predispensed reagent cartridges supplied in the Maxwell® 16 LEV Plant RNA Kit. The instrument can process from 1 to 16 samples in under an hour, and the purified RNA can be used directly in downstream applications including real-time RT-qPCR, gel electrophoresis, microarrays and sequencing (e.g., NGS and Sanger).

Consistent processing and higher yields mean less re-isolation of samples, reducing costs and increasing the reliability of your results.
No organic extraction and minimal preprocessing required.

Purifies High-Quality RNA from Arabidopsis, Soybean and Corn

Yield of RNA from corn, soybean, arabidopsis using Maxwell Plant RNA kit — RNA was extracted from 40mg of Arabidopsis, corn and soybean leaf tissues (n=6 for each sample type) using the Maxwell® RSC Plant RNA Kit and eluted in 50µl. Eluted RNA was quantified using the QuantiFluor® RNA Dye and the Quantus™ Fluorometer.

Purity of RNA isolated from corn, soybean, arabidopsis using the Maxwell 16 Plant RNA Kit — RNA was extracted from 40mg of fresh leaf tissues (n=4 for each sample type) using the Maxwell® 16 Plant RNA kit and eluted in 50µl. Yield was determined by spectroscopy. A₂₆₀/A₂₈₀ ratios above 1.8, and A₂₆₀/A₂₃₀ ratios between 1.8 and 2.4 were achieved for all samples.

Purified RNA Performs Well In RT-PCR

RNA Yield and Performance

RNA yield from corn, soybean and arabidopsis using the Maxwell 16 Plant RNA Kit — RNA was extracted from 40mg of plant leaf tissue from the indicated species, eluted in 50µl and quantitated by RT-qPCR using species-specific primer/probe sets. The 1:10 dilution for all three species indicates that there is no inhibition based on volume input.

No Inhibition in RT-PCR

No inhibition of RT PCR using RNA extracted with the Maxwell 16 Plant RNA Kit — Inhibition of amplification of extracted RNA: Comparison to an Internal Positive Control. RNA samples (n=4), purified from leaves of the indicated plants, were added to a TaqMan® Exogenous Internal Positive Control (IPC) reaction. A ΔCt value of zero indicates no inhibition, and a ΔCt of two or below is considered acceptable. The observed ΔCt values for the Maxwell® 16 LEV Plant RNA samples indicate minimal inhibition.

Protocolos

Complete Protocol

Maxwell® 16 LEV Plant RNA Kit Technical ManualPDF (810 KB) – English

Protocolos Rápidos

Maxwell® 16 LEV Plant RNA Kit Quick Protocol, FB170PDF (237 KB) – English

Contenido

Item	Part #	Presentación	Available Separately
1-Thioglycerol	A208B	1 × 900μl
Maxwell® RSC Cartridge (RSCO)	AS174A	48 × 1 each
LEV Plungers	AS610A	1 × 50/pk	View Product
Elution Tubes (0.5ml)	AS620A	1 × 50/pk	View Product
Blue Dye	C888A	1 × 50μl
Lysis Buffer	MC501C	1 × 20ml
Nuclease-Free Water	P119C	1 × 25ml	View Product
Homogenization Solution	Z305H	1 × 30ml
DNase I (lyophilized)	Z358A	2 × 1 vial	View Product

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Recursos

Software y Firmware

Maxwell® 16

Usado con frecuencia junto con

Quantus™ Fluorometer and NGS Starter Package

Dos canales de fluorescencia para la cuantificación de ácidos nucleicos y proteínas. Disponible en paquete para aplicaciones NGS.

E6150, E5150

QuantiFluor® RNA System

Cuantificación fluorescente de pequeñas cantidades de ARN en solución.

E3310

Maxwell® 16 LEV Plant RNA Kit

Faster RNA Purification, Less Hands-On Time and More Efficient Workflows

Purifies High-Quality RNA from Arabidopsis, Soybean and Corn

Purified RNA Performs Well In RT-PCR

RNA Yield and Performance

No Inhibition in RT-PCR

Protocolos

Complete Protocol

Protocolos Rápidos

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Recursos

Software y Firmware

Productos Similares

Maxwell® RSC Instrument

Maxwell® RSC Plant RNA Kit

Maxwell® RSC SimplyRNA Cells Kit

Usado con frecuencia junto con

Quantus™ Fluorometer and NGS Starter Package

QuantiFluor® RNA System

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