The ability to extract RNA from FFPE samples allows correlation of disease state and tissue morphology with gene expression. Extraction of RNA from FFPE tissues is challenging because the formalin fixation process cross-links proteins and nucleic acids and introduces modifications that can inhibit subsequent analysis of RNA isolated from FFPE tissues.
The ReliaPrep™ FFPE Total RNA Miniprep System optimizes lysis conditions to reverse these RNA modifications without the need for overnight digestion. The system also uses a deparaffinization method that does not rely on xylene or other organic solvents. By eliminating the washing steps involved in xylene deparaffinization, this method can help retain small RNA fragments.
The protocol is complete in 1.5 hours with minimal hands-on time.