M-MLV Reverse Transcriptase

RNA-Dependent DNA Polymerase for cDNA Synthesis

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Advantages

Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl₂, 50mM DTT.
May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

View M-MLV Citations

Protocolos

Complete Protocol

M-MLV Reverse Transcriptase ProtocolPDF (238 KB) – English

Contenido

Item	Part #	Presentación
M-MLV Reverse Transcriptase	M170A	1 × 10,000u
M-MLV RT 5X Buffer	M531A	1 × 1ml

SDS

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Certificado de Análisis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Condiciones de Almacenaje

Contenido

Item	Part #	Presentación
M-MLV Reverse Transcriptase	M170B	1 × 50,000u
M-MLV RT 5X Buffer	M531A	2 × 1ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Condiciones de Almacenaje

Contenido

Item	Part #	Presentación
M-MLV RT 5X Buffer	M531A	2 × 1ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Condiciones de Almacenaje

Recursos

Artículos

G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker, promotes proliferation and invasion of triple negative breast cancer

Menciones

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M-MLV Reverse Transcriptase

RNA-Dependent DNA Polymerase for cDNA Synthesis

RNA-Dependent DNA Polymerase for cDNA Synthesis

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Recursos

Artículos

Menciones

Access RT-PCR System

Reverse Transcription System

M-MLV Reverse Transcriptase, RNase H Minus

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

AMV Reverse Transcriptase

dNTP Mix

Random Primers

Oligo(dT)₁₅ Primer

RNasin® Ribonuclease Inhibitor

SV Total RNA Isolation System

ReliaPrep™ FFPE Total RNA Miniprep System

Maxwell® RSC SimplyRNA Cells Kit

M-MLV Reverse Transcriptase

RNA-Dependent DNA Polymerase for cDNA Synthesis

RNA-Dependent DNA Polymerase for cDNA Synthesis

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Recursos

Artículos

Menciones

Productos Similares

Access RT-PCR System

Reverse Transcription System

M-MLV Reverse Transcriptase, RNase H Minus

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

AMV Reverse Transcriptase

Usado con frecuencia junto con

dNTP Mix

Random Primers

Oligo(dT)15 Primer

RNasin® Ribonuclease Inhibitor

SV Total RNA Isolation System

ReliaPrep™ FFPE Total RNA Miniprep System

Maxwell® RSC SimplyRNA Cells Kit

Americas

Pacific Asia

Europe

Oligo(dT)₁₅ Primer