Polyclonal secondary antibody raised in donkey, immunoaffinity-purified using immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme.
Donkey Anti-Goat IgG, HRP Conjugate is a secondary antibody developed in donkeys against goat IgG. This antibody shows reactivity to goat and sheep IgG but minimal cross-reactivity to rabbit and mouse IgG. For Western blot applications with chromogenic detection, use the secondary antibody at a starting dilution of 1:10,000. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted) is supplied as a 1mg/ml solution. The optimum concentration of secondary antibody depends on the application and will need to be empirically determined.
We can readily accommodate large-scale custom orders. Please inquire at: www.promega.com/custom/
Applications
- Western blotting.
- Enzyme-linked immunosorbent assay (ELISA).
- Dot blotting.
References
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- Massaad, C.A., Washington, T.M., Pautler, R.G. and Klann, E. (2009) Overexpression of SOD-2 reduces hippocampal superoxide and prevents memory deficits in a mouse model of Alzheimer's disease. Proc. Natl. Acad. Sci. USA 106, 13576–81.
- Berkowitz, O., Jost, R., Pollmann, S. and Masle, J. (2008) Characterization of TCTP, the translationally controlled tumor protein, from Arabidopsis thaliana. Plant Cell 20, 3430–47.
- Robertson, J.B., Stowers, C.C., Boczko, E. and Johnson, C.H. (2008) Real-time luminescence monitoring of cell-cycle and respiratory oscillations in yeast. Proc. Natl. Acad. Sci. USA 105, 17988–93.