Calpain-Glo™ Protease Assay

G8501_Calpain-Glo--Protease-Assay-10ml_3
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Homogeneous, Luminescent Assay that Measures Calpain 1 (μ) and 2 (m) Activities

  • Simple "add-mix-measure" protocol
  • Maximum sensitivity while enzyme is still active
  • 1,000X more sensitive than fluorometric assays

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Catalog number selected: G8501

$ 413.00
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Calpain-Glo™ Protease Assay
10ml
$ 413.00
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Simple Protocol Offers Fast, Sensitive Results

The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities. Calpains are a family of calcium-activated cysteine proteases involved in cleaving a wide variety of proteins. Calpains modulate the biological activities of their substrates via limited proteolysis.

The Calpain-Glo™ Protease Assay provides a succinyl, proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimized for calpain and luciferase activities. The addition of the calpain reagent in an "add-mix-measure" format results in calpain cleavage of the substrate and rapid development of a "glow-type" luminescent signal produced by the luciferase reaction. The signal is proportional to the amount of calpain activity present. The assay is designed for use with purified enzyme preparations.

  • Designed for measuring calpain activity and/or inhibition in a multiwell format and easy to automate.
  • Well suited for rapidly autolysed enzymes like calpain; maximum sensitivity is reached in as little as 10 minutes, while the enzyme is fully active.
  • Up to 1,000 times more sensitive than competitive fluorometric assays. The luminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-background readings. The assay is linear over 4 logs of calpain concentration.
Reference
  1. Moldoveanu, T. et al. (2002) Cell 108, 649–60.
5108MA-W
Sensitivity of the Calpain-Glo™ Protease Assay compared to fluorescent assays.

Especificaciones

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Contenido

Item Part # Presentación

Calpain-Glo™ Buffer

G852A 1 × 10ml

Suc-LLVY-Glo™ Substrate

G856A 1 × 100μl

Luciferin Detection Reagent

V859A 1 × 1 each

Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

See Protocol for storage recommendations.

Especificaciones

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Contenido

Item Part # Presentación

Calpain-Glo™ Buffer

G852B 1 × 50ml

Suc-LLVY-Glo™ Substrate

G856B 1 × 500μl

Luciferin Detection Reagent

V859B 1 × 1 each

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

See Protocol for storage recommendations.

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