N274 NanoLuc CRBN This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N274 NanoLuc CRBN sequence reference points: Base pairs 5959 CMV immediate-early enhancer/promoter 1-742 T7 RNA Polymerase promoter (-17 to +3) 1033-1052 NanoLuc-CRBN Fusion Coding Region 1065-2930 Neo-Kan Resistance 3910-4704 ---- 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGCT CACCATGGTC TTCACACTCG AAGATTTCGT TGGGGACTGG 1101 CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC AGGGAGGTGT 1151 GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA 1201 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC 1251 CCGTATGAAG GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT 1301 TAAGGTGGTG TACCCTGTGG ATGATCATCA CTTTAAGGTG ATCCTGCACT 1351 ATGGCACACT GGTAATCGAC GGGGTTACGC CGAACATGAT CGACTATTTC 1401 GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA AGATCACTGT 1451 AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA 1501 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC 1551 TGGCGGCTGT GCGAACGCAT TCTGGCGGGC TCGAGCGGCG CGATCGCCAT 1601 GGCCGGCGAA GGAGATCAGC AGGACGCTGC GCACAACATG GGCAACCACC 1651 TGCCGCTCCT GCCTGCAGAG AGTGAGGAAG AAGATGAAAT GGAAGTTGAA 1701 GACCAGGATA GTAAAGAAGC CAAAAAACCA AACATCATAA ATTTTGACAC 1751 CAGTCTGCCG ACATCACATA CATACCTAGG TGCTGATATG GAAGAATTTC 1801 ATGGCAGGAC TTTGCACGAT GACGACAGCT GTCAGGTGAT TCCAGTTCTT 1851 CCACAAGTGA TGATGATCCT GATTCCCGGA CAGACATTAC CTCTTCAGCT 1901 TTTTCACCCT CAAGAAGTCA GTATGGTGCG GAATTTAATT CAGAAAGATA 1951 GAACCTTTGC TGTTCTTGCA TACAGCAATG TACAGGAAAG GGAAGCACAG 2001 TTTGGAACAA CAGCAGAGAT ATATGCCTAT CGAGAAGAAC AGGATTTTGG 2051 AATTGAGATA GTGAAAGTGA AAGCAATTGG AAGACAAAGG TTCAAAGTCC 2101 TTGAGCTAAG AACACAGTCA GATGGAATCC AGCAAGCTAA AGTGCAAATT 2151 CTTCCCGAAT GTGTGTTGCC TTCAACCATG TCTGCAGTTC AATTAGAATC 2201 CCTCAATAAG TGCCAGATAT TTCCTTCAAA ACCTGTCTCA AGAGAAGACC 2251 AATGTTCATA TAAATGGTGG CAGAAATACC AGAAGAGAAA GTTTCATTGT 2301 GCAAATCTAA CTTCATGGCC TCGCTGGCTG TATTCCTTAT ATGATGCTGA 2351 GACCTTAATG GACAGAATCA AGAAACAGCT ACGTGAATGG GATGAAAATC 2401 TAAAAGATGA TTCTCTTCCT TCAAATCCAA TAGATTTTTC TTACAGAGTA 2451 GCTGCTTGTC TTCCTATTGA TGATGTATTG AGAATTCAGC TCCTTAAAAT 2501 TGGCAGTGCT ATCCAGCGAC TTCGCTGTGA ATTAGACATT ATGAATAAAT 2551 GTACTTCCCT TTGCTGTAAA CAATGTCAAG AAACAGAAAT AACAACCAAA 2601 AATGAAATAT TCAGTTTATC CTTATGTGGG CCGATGGCAG CTTATGTGAA 2651 TCCTCATGGA TATGTGCATG AGACACTTAC TGTGTATAAG GCTTGCAACT 2701 TGAATCTGAT AGGCCGGCCT TCTACAGAAC ACAGCTGGTT TCCTGGGTAT 2751 GCCTGGACTG TTGCCCAGTG TAAGATCTGT GCAAGCCATA TTGGATGGAA 2801 GTTTACGGCC ACCAAAAAAG ACATGTCACC TCAAAAATTT TGGGGCTTAA 2851 CGCGATCTGC TCTGTTGCCC ACGATCCCAG ACACTGAAGA TGAAATAAGT 2901 CCAGACAAAG TAATACTTTG CTTGGTTTAA ACGAATTCGG GCTCGGTACC 2951 CGGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTGA TCCGGCTGCT 3001 AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA 3051 ACTAGCATAA CCCCTTGGGG CGGCCGCTTC GAGCAGACAT GATAAGATAC 3101 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT 3151 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT 3201 GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT 3251 CAGGGGGAGA TGTGGGAGGT TTTTTTAAGC AAGTAAAACC TCTACAAATG 3301 TGGTAAAATC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCTGATGC 3351 GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATACGCGGA 3401 TCTGCGCAGC ACCATGGCCT GAAATAACCT CTGAAAGAGG AACTTGGTTA 3451 GGTACCTTCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG 3501 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 3551 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 3601 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 3651 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT 3701 CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 3751 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 3801 CCTAGGCTTT TGCAAAAAGC TTAATTAACT GTTGACAATT AATCATCGGC 3851 ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCCAGG 3901 AGGCAGATCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC 3951 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 4001 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 4051 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC 4101 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC 4151 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 4201 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC 4251 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 4301 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 4351 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG 4401 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG 4451 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 4501 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT 4551 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG 4601 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 4651 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT 4701 CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT 4751 GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 4801 GTTGGTTTTT TGTGTGAATC GATAGCGATA AGGATCCGCT CATGCGATGA 4851 TAAGCTGTCA AACATGAGAA TTACAACTTA TATCGTATGG GGCTGACTTC 4901 AGGTGCTACA TTTGAAGAGA TAAATTGCAC TGAAATCTAG AAATATTTTA 4951 TCTGATTAAT AAGATGATCT TCTTGAGATC GTTTTGGTCT GCGCGTAATC 5001 TCTTGCTCTG AAAACGAAAA AACCGCCTTG CAGGGCGGTT TTTCGAAGGT 5051 TCTCTGAGCT ACCAACTCTT TGAACCGAGG TAACTGGCTT GGAGGAGCGC 5101 AGTCACCAAA ACTTGTCCTT TCAGTTTAGC CTTAACCGGC GCATGACTTC 5151 AAGACTAACT CCTCTAAATC AATTACCAGT GGCTGCTGCC AGTGGTGCTT 5201 TTGCATGTCT TTCCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 5251 CAGCGGTCGG ACTGAACGGG GGGTTCGTGC ATACAGTCCA GCTTGGAGCG 5301 AACTGCCTAC CCGGAACTGA GTGTCAGGCG TGGAATGAGA CAAACGCGGC 5351 CATAACAGCG GAATGACACC GGTAAACCGA AAGGCAGGAA CAGGAGAGCG 5401 CACGAGGGAG CCGCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 5451 GGTTTCGCCA CCACTGATTT GAGCGTCAGA TTTCGTGATG CTTGTCAGGG 5501 GGGCGGAGCC TATGGAAAAA CGGCTTTGCC GCGGCCCTCT CACTTCCCTG 5551 TTAAGTATCT TCCTGGCATC TTCCAGGAAA TCTCCGCCCC GTTCGTAAGC 5601 CATTTCCGCT CGCCGCAGTC GAACGACCGA GCGTAGCGAG TCAGTGAGCG 5651 AGGAAGCGGA ATATATCCTG TATCACATAT TCTGCTGACG CACCGGTGCA 5701 GCCTTTTTTC TCCTGCCACA TGAAGCACTT CACTGACACC CTCATCAGTG 5751 CCAACATAGT AAGCCAGTAT ACACTCCGCT AGCGCTGATG TCCGGCGGTG 5801 CTTTTGCCGT TACGCACCAC CCCGTCATGA GATTACGCGT TTTAGCGGTG 5851 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC 5901 CATCGAGATA AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA 5951 TCGAGATCT Sequence file prepared 9/4/2020 by Feature database and feature finder version 1.0.5.0.