Rabbit Reticulocyte Lysate, Untreated

Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA. Untreated Lysate is prepared from New Zealand white rabbits in the same manner as treated lysates with the exception that it is not treated with micrococcal nuclease.

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Applications

Isolation of the cellular components necessary for protein synthesis.
Translation of exogenous mRNA, after further optimization, including the addition of energy-regenerating systems, hemin, endogenous tRNA and micrococcal nuclease to remove endogenous mRNA.

Reference

Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.

Protocolos

Complete Protocol

Rabbit Reticulocyte Lysate Technical ManualPDF (436 KB) – English

Contenido

Item	Part #	Presentación
Rabbit Reticulocyte Lysate, Untreated	L415A	1 × 1,000μl

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Recursos

Menciones

Carbohydrates act as sorting determinants in ER-associated degradation of tryosinase.
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2004 J. Cell Sci.
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Usado con frecuencia junto con

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Detección no radiactiva de proteínas sintetizadas in vitro.

L5070

Rabbit Reticulocyte Lysate, Untreated

Characterize Protein Products, Study Transcriptional and Translational Control