S30 T7 High-Yield Protein Expression System

With the S30 T7 High-Yield Protein Expression System you can express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for protein expression. This results in greater stability and enhanced expression of target proteins.

Detect expressed proteins by Coomassie® staining or incorporation of a fluorescence or biotinylated modified tRNA. For more information, see the Protocols & Applications Guide.

• Protein:protein interactions
• Protein:DNA interactions
• Enzyme activity
• Antibody production