All the tools you need in one bundle to start cell-free expression and detection of your proteins. Use the system for in vitro analysis of protein:protein or protein:nucleic acid interactions, or simply verify the ability of your clone to express protein. Purchase this special bundle, and get the popular TnT® T7 Quick Coupled Transcription/Translation System and Transcend™ Translation Detection System, and receive two cell-free expression-qualified expression vectors, pTnT™ and pCMVTnT™ Vectors, at no extra cost. Each bundle includes:
TnT® T7 Quick Coupled Transcription/Translation System: Our most popular cell-free translation system—a simple one-hour, one-tube reaction. Requires only a protein coding sequence downstream of a T7 RNA polymerase promoter to produce protein. Produced protein may be used in a variety of applications including pull-downs, immunoprecipitations and gel shift assays. TnT® T7 Quick Coupled Transcription/Translation System Technical Manual #TM045.
Transcend™ Translation Detection Systems: A simple addition of the Transcend biotin-labeled lysine tRNA to the TnT® T7 Quick reaction provides a simple means of tagging a protein for easy detection. Detect proteins through simple Western blotting techniques with colorimetric techniques. Transcend™ Translation Detection Systems Technical Bulletin #TB182.
pTnT™ Vector: Specifically designed to work with the TnT® Systems with added features to enhance cell-free expression. pTnT™ Vector Technical Bulletin #TB304.
pCMVTnT™ Vector: Specifically designed to work with the TnT® Systems with added features to enhance cell-free expression. Go from cell-free expression to mammalian expression directly with built-in CMV promoter. pCMVTnT™ Vector Technical Bulletin #TB305.
Applications
- GST pull-downs.
- Gel shift assays (view an animation).
- Co-immunoprecipitations.
- Characterization of protein modifications.
- Protein:RNA interactions.
- Protein activity studies.
- Conformation of gene products.