N203 LgBiT-PRKAR2A Control Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N203 LgBiT-PRKAR2A Control Vector Vector sequence reference points: Base pairs 5715 HSV-TK promoter 1-753 LgBiT-PRKAR2A 802-2544 Sv40 late polyadenylation signal 2704-2925 SV40 enhancer and early promoter 3024-3442 SV40 Enhancer 3097-3333 (Reverse) Sv40 Min Ori 3340-3405 EM7 bacterial promoter 3450-3516 Neo-Kan Resistance 3530-4324 Synthetic PolyAdenylation Signal Sequence 4388-4436 ColE1-derived plasmid replication origin 4672-4708 ---- 1 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT 51 GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 101 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT 151 CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG 201 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT 251 GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG 301 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC 351 TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG 401 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC 451 CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT 501 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC 551 TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG 601 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAAGTCGA 651 ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT 701 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT 751 TAAAAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCGC TAGCGCTCAC 801 CATGGTCTTC ACACTCGAAG ATTTCGTTGG GGACTGGGAA CAGACAGCCG 851 CCTACAACCT GGACCAAGTC CTTGAACAGG GAGGTGTGTC CAGTTTGCTG 901 CAGAATCTCG CCGTGTCCGT AACTCCGATC CAAAGGATTG TCCGGAGCGG 951 TGAAAATGCC CTGAAGATCG ACATCCATGT CATCATCCCG TATGAAGGTC 1001 TGAGCGCCGA CCAAATGGCC CAGATCGAAG AGGTGTTTAA GGTGGTGTAC 1051 CCTGTGGATG ATCATCACTT TAAGGTGATC CTGCCCTATG GCACACTGGT 1101 AATCGACGGG GTTACGCCGA ACATGCTGAA CTATTTCGGA CGGCCGTATG 1151 AAGGCATCGC CGTGTTCGAC GGCAAAAAGA TCACTGTAAC AGGGACCCTG 1201 TGGAACGGCA ACAAAATTAT CGACGAGCGC CTGATCACCC CCGACGGCTC 1251 CATGCTGTTC CGAGTAACCA TCAACAGTGG GAGCTCCGGT GGTGGCGGGA 1301 GCGGAGGTGG AGGCTCGAGC GGTGCGATCG CCATGAGCCA CATCCAGATC 1351 CCGCCGGGGC TCACGGAGCT GCTGCAGGGC TACACGGTGG AGGTGCTGCG 1401 ACAGCAGCCG CCTGACCTCG TCGAATTCGC AGTGGAGTAC TTCACCCGCC 1451 TGCGCGAGGC CCGCGCCCCA GCCTCAGTCC TGCCCGCCGC CACCCCACGC 1501 CAGAGCCTGG GCCACCCCCC GCCAGAACCC GGCCCGGACC GTGTCGCCGA 1551 CGCCAAAGGG GACAGCGAGT CGGAGGAGGA CGAGGACTTG GAAGTTCCAG 1601 TTCCTAGCAG ATTTAATAGA CGAGTATCAG TCTGTGCTGA GACCTATAAC 1651 CCTGATGAGG AAGAGGAAGA TACAGATCCA AGGGTGATTC ATCCTAAAAC 1701 TGATGAACAG AGATGCAGAC TTCAGGAAGC TTGCAAAGAT ATTCTCCTTT 1751 TCAAAAATCT TGATCAGGAA CAGCTTTCTC AAGTTCTCGA TGCCATGTTT 1801 GAAAGGATAG TCAAAGCTGA TGAGCATGTC ATTGACCAAG GAGATGATGG 1851 AGACAACTTT TATGTCATAG AACGGGGAAC TTATGACATT TTAGTAACAA 1901 AAGATAATCA AACCCGCTCT GTTGGTCAAT ATGACAACCG TGGCAGTTTT 1951 GGAGAACTAG CTCTGATGTA CAACACCCCG AGAGCTGCTA CCATTGTTGC 2001 TACCTCAGAA GGCTCCCTTT GGGGACTGGA CCGGGTGACT TTTAGAAGAA 2051 TCATAGTGAA AAATAATGCA AAGAAGAGGA AGATGTTTGA ATCATTTATT 2101 GAGTCTGTGC CCCTCCTTAA ATCACTAGAG GTGTCAGAAC GAATGAAGAT 2151 TGTGGATGTA ATAGGAGAGA AGATCTATAA GGATGGAGAA CGCATAATCA 2201 CTCAGGGTGA AAAGGCTGAT AGCTTTTACA TCATAGAGTC TGGCGAAGTG 2251 AGCATCTTGA TTAGAAGCAG GACTAAATCA AACAAGGATG GTGGGAACCA 2301 GGAGGTCGAG ATTGCCCGCT GCCATAAGGG GCAGTACTTT GGAGAGCTTG 2351 CCCTGGTCAC CAACAAACCC AGAGCTGCCT CAGCTTATGC AGTTGGAGAT 2401 GTCAAATGCT TAGTTATGGA TGTACAAGCA TTCGAGAGGC TTCTGGGGCC 2451 CTGCATGGAC ATCATGAAGA GGAACATCTC ACACTATGAG GAACAGCTGG 2501 TGAAGATGTT TGGCTCCAGC GTGGATCTGG GCAACCTCGG GCAGGTTTAA 2551 ACGAATTCGG GCTCGGTACC CGGGGATCCT CTAGAGTCGA CCTGCAGGCA 2601 TGCAAGCTGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT 2651 GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CGGCCGCTTC 2701 GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 2751 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT 2801 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA 2851 TTCATTTTAT GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT TTTTTTAAGC 2901 AAGTAAAACC TCTACAAATG TGGTAAAATC GAATTTTAAC AAAATATTAA 2951 CGCTTACAAT TTCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA 3001 TTTCACACCG CATACGCGGA TCTGCGCAGC ACCATGGCCT GAAATAACCT 3051 CTGAAAGAGG AACTTGGTTA GGTACCTTCT GAGGCGGAAA GAACCAGCTG 3101 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 3151 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA 3201 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 3251 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 3301 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 3351 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 3401 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTAATTAACT 3451 GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA 3501 GGTGAGGAAC TAAACCCAGG AGGCAGATCA TGATTGAACA AGATGGATTG 3551 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG 3601 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG 3651 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 3701 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG 3751 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 3801 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 3851 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA 3901 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA 3951 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 4001 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT 4051 CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG 4101 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 4151 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC 4201 TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC 4251 TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 4301 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC 4351 GACCAAGCGA CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT 4401 TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGCGATA 4451 AGGATCCTCT TTGCGCTTGC GTTTTCCCTT GTCCAGATAG CCCAGTAGCT 4501 GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACCCGG 4551 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 4601 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 4651 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 4701 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 4751 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 4801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 4851 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 4901 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 4951 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 5001 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 5051 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 5101 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 5151 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 5201 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 5251 AAAAGGATTT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 5301 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 5351 AGGATCTTCA CCTAGATCCT TTTATAGTCC GGAAATACAG GAACGCACGC 5401 TGGATGGCCC TTCGCTGGGA TGGTGAAACC ATGAAAAATG GCAGCTTCAG 5451 TGGATTAAGT GGGGGTAATG TGGCCTGTAC CCTCTGGTTG CATAGGTATT 5501 CATACGGTTA AAATTTATCA GGCGCGATTG CGGCAGTTTT TCGGGTGGTT 5551 TGTTGCCATT TTTACCTGTC TGCTGCCGTG ATCGCGCTGA ACGCGTTTTA 5601 GCGGTGCGTA CAATTAAGGG ATTATGGTAA ATCCACTTAC TGTCTGCCCT 5651 CGTAGCCATC GAGATAAACC GCAGTACTCC GGCCACGATG CGTCCGGCGT 5701 AGAGGATCGA GATCT Sequence file prepared 11/20/2015 by Feature database and feature finder version 1.0.4.2.