N194 pFN33K LgBiT TK-neo Flexi Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N194 pFN33K LgBiT TK-neo Flexi Vector Vector sequence reference points: Base pairs 4862 HSV-TK promoter 1-753 LgBiT 802-1278 SgfI Site 1324-1331 Barnase coding region 1355-1690 PmeI site 1692-1699 Sv40 late polyadenylation signal 1851-2072 SV40 enhancer and early promoter 2171-2589 SV40 Enhancer 2244-2480 (Reverse) Sv40 Min Ori 2487-2552 EM7 bacterial promoter 2597-2663 Neo-Kan Resistance 2677-3471 Synthetic PolyAdenylation Signal Sequence 3535-3583 ColE1-derived plasmid replication origin 3819-3855 ---- 1 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT 51 GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 101 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT 151 CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG 201 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT 251 GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG 301 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC 351 TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG 401 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC 451 CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT 501 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC 551 TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG 601 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAAGTCGA 651 ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT 701 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT 751 TAAAAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCGC TAGCGCTCAC 801 CATGGTCTTC ACACTCGAAG ATTTCGTTGG GGACTGGGAA CAGACAGCCG 851 CCTACAACCT GGACCAAGTC CTTGAACAGG GAGGTGTGTC CAGTTTGCTG 901 CAGAATCTCG CCGTGTCCGT AACTCCGATC CAAAGGATTG TCCGGAGCGG 951 TGAAAATGCC CTGAAGATCG ACATCCATGT CATCATCCCG TATGAAGGTC 1001 TGAGCGCCGA CCAAATGGCC CAGATCGAAG AGGTGTTTAA GGTGGTGTAC 1051 CCTGTGGATG ATCATCACTT TAAGGTGATC CTGCCCTATG GCACACTGGT 1101 AATCGACGGG GTTACGCCGA ACATGCTGAA CTATTTCGGA CGGCCGTATG 1151 AAGGCATCGC CGTGTTCGAC GGCAAAAAGA TCACTGTAAC AGGGACCCTG 1201 TGGAACGGCA ACAAAATTAT CGACGAGCGC CTGATCACCC CCGACGGCTC 1251 CATGCTGTTC CGAGTAACCA TCAACAGTGG GAGCTCCGGT GGTGGCGGGA 1301 GCGGAGGTGG AGGCTCGAGC GGTGCGATCG CCATGGAATA AGTAAGGAAT 1351 CCACATGGCA CAGGTTATCA ACACGTTTGA CGGGGTTGCG GATTATCTTC 1401 AGACATATCA TAAGCTACCT GATAATTACA TTACAAAATC AGAAGCACAA 1451 GCCCTCGGCT GGGTGGCATC AAAAGGGAAC CTTGCAGACG TCGCTCCGGG 1501 GAAAAGCATC GGCGGAGACA TCTTCTCAAA CAGGGAAGGC AAACTCCCGG 1551 GCAAAAGCGG ACGAACATGG CGTGAAGCGG ATATTAACTA TACATCAGGC 1601 TTCAGAAATT CAGACCGGAT TCTTTACTCA AGCGACTGGC TGATTTACAA 1651 AACAACGGAC CATTATCAGA CCTTTACAAA AATCAGATAA TGTTTAAACG 1701 AATTCGGGCT CGGTACCCGG GGATCCTCTA GAGTCGACCT GCAGGCATGC 1751 AAGCTGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT 1801 GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCGG CCGCTTCGAG 1851 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG 1901 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT 1951 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 2001 ATTTTATGTT TCAGGTTCAG GGGGAGATGT GGGAGGTTTT TTTAAGCAAG 2051 TAAAACCTCT ACAAATGTGG TAAAATCGAA TTTTAACAAA ATATTAACGC 2101 TTACAATTTC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT 2151 CACACCGCAT ACGCGGATCT GCGCAGCACC ATGGCCTGAA ATAACCTCTG 2201 AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG GCGGAAAGAA CCAGCTGTGG 2251 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 2301 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT 2351 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 2401 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC 2451 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 2501 ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 2551 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA ATTAACTGTT 2601 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT 2651 GAGGAACTAA ACCCAGGAGG CAGATCATGA TTGAACAAGA TGGATTGCAC 2701 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 2751 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 2801 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 2851 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 2901 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 2951 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 3001 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 3051 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 3101 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 3151 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 3201 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 3251 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 3301 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 3351 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 3401 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 3451 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC 3501 CAAGCGACGC CCAACCTGCC ATCACGATGG CCGCAATAAA ATATCTTTAT 3551 TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGCGATAAGG 3601 ATCCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC AGTAGCTGAC 3651 ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC TACCCGGTAT 3701 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 3751 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 3801 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 3851 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 3901 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 3951 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 4001 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 4051 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 4101 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 4151 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 4201 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 4251 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 4301 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 4351 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 4401 AGGATTTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 4451 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 4501 ATCTTCACCT AGATCCTTTT ATAGTCCGGA AATACAGGAA CGCACGCTGG 4551 ATGGCCCTTC GCTGGGATGG TGAAACCATG AAAAATGGCA GCTTCAGTGG 4601 ATTAAGTGGG GGTAATGTGG CCTGTACCCT CTGGTTGCAT AGGTATTCAT 4651 ACGGTTAAAA TTTATCAGGC GCGATTGCGG CAGTTTTTCG GGTGGTTTGT 4701 TGCCATTTTT ACCTGTCTGC TGCCGTGATC GCGCTGAACG CGTTTTAGCG 4751 GTGCGTACAA TTAAGGGATT ATGGTAAATC CACTTACTGT CTGCCCTCGT 4801 AGCCATCGAG ATAAACCGCA GTACTCCGGC CACGATGCGT CCGGCGTAGA 4851 GGATCGAGAT CT Sequence file prepared 11/20/2015 by Feature database and feature finder version 1.0.4.2.