MagneGST™ Pull-Down System

The MagneGST™ Pull-Down System is designed to detect protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT® Systems. Prey protein synthesized in the TnT® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively labeled methionine in the TnT® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating the supplied non-radioactive methionine in the TnT® reaction and detecting by Western blotting with protein-specific antibodies (see figure).

Note: This product requires the use of a magnetic stand, which is not included.

For more information, see the Protocols & Applications Guide.

Application

GST pull-down assays for the analysis of protein:protein interactions.

Representation of pull-down assay using bacterial expression of bait protein and TNT® cell-free system for the expression of the prey protein. — **Representation of pull-down assay using bacterial expression of bait protein and TnT® cell-free system for the expression of the prey protein.**

Protocolos

Complete Protocol

MagneGST™ Pull-Down System Technical ManualPDF (483 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
Methionine	L118A	1 × 50μl	1mM
RQ1 RNase-Free DNase	M610A	1 × 1,000u		View Product
Nuclease-Free Water	P119A	1 × 1,250μl		View Product
MagneGST™ Cell Lysis Reagent	V481B	1 × 60ml
MagneGST™ Glutathione Particles	V861A	1 × 4ml		View Product
MagneGST™ Binding/Wash Buffer	V870B	2 × 200ml
TnT® T7 Quick Master Mix	L520A	8 × 200μl

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

See Protocol for detailed storage recommendations.

Productos Similares

TnT® Quick Coupled Transcription/Translation System

Sistema de transcripción/traducción acoplado que produce cantidades significativas de proteína para aplicaciones que incluyen GST pull-downs y ensayos de shift en gel en 60-90 minutos.

L1170, L1171, L2080, L2081

MagneGST™ Protein Purification System

Immobilized paramagnetic particles isolate GST-tagged protein directly from crude or cleared lysate by either a manual or automated procedure, with a magnetic stand.

V8600, V8603

Usado con frecuencia junto con

MagneSphere® Technology Magnetic Separation Stands

Imanes potentes de tierras raras para aplicaciones de separación magnética.

Z5331, Z5332, Z5333, Z5341, Z5342, Z5343