QuantiFluor® ONE dsDNA System

Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nm Ex/531nm Em) in an automation-friendly, "add-and-read" format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It's as easy to use as NanoDrop® absorbance-based methods but much more sensitive for low-concentration samples.

QuantiFluor Nanodrop Spectrophotometer DNA Extraction

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples and better or equal sensitivity performance when compared to Qubit® dsDNA HS. QuantiFluor® ONE Dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay).

DNA Extraction & Purification with Better Sensitivity

	Sample*	Assay
QuantiFluor® ONE dsDNA System	0.2–400ng/µl	0.2–400ng
Qubit® dsDNA HS	0.2–100ng/µl	0.2–100ng
Qubit® dsDNA BR	2–1,000ng/µl	2–1,000ng

*Based on 1µl sample input per assay.

DNA Quantitation Assay Sensitivity and Linearity

The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lambda or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2–400ng/µl (1µl sample in a 200µl assay).

Easy Setup on Microplate or Single-Tube Fluorometers

System includes all the necessary reagents to quickly set up and quantitate dsDNA and is easy to set up on microplate or single-tube fluorometers, including the following Promega fluorometers.

Quantus Nucleic Acid Quantification Fluorometer

Quantus™ Fluorometer

(Use Blue Channel Ex 460nm, Em 515–575nm)

Discover Nanodrop Spectrophotometer DNA Extraction

GloMax® Discover

Explorer DNA Purification & Quantification Kit

GloMax® Explorer

How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wieczorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

Fluorescence vs qPCR

Protocolos

Complete Protocol

QuantiFluor® ONE dsDNA SystemPDF (1672 KB) – English

Protocolos Rápidos

QuantiFluor ONE dsDNA System Quick Protocol FB200PDF (823 KB) – English

Contenido

Item	Part #	Presentación	Available Separately
QuantiFluor® ONE dsDNA Dye	E489A	1 × 20ml	View Product
QuantiFluor® ONE Lambda DNA	E490B	1 × 80μg
1X TE Buffer (pH 7.5)	E497B	1 × 10ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Store QuantiFluor® ONE dsDNA Dye and Lambda DNA at –30°C to +10°C. Store 1X TE Buffer at –30°C to +30°C.

Patentes y Exclusiones

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Available Separately
QuantiFluor® ONE dsDNA Dye	E489A	5 × 20ml	View Product
QuantiFluor® ONE Lambda DNA	E490A	1 × 400μg
1X TE Buffer (pH 7.5)	E497A	1 × 25ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Store QuantiFluor® ONE dsDNA Dye and Lambda DNA at –30°C to +10°C. Store 1X TE Buffer at –30°C to +30°C.

Patentes y Exclusiones

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Recursos

Menciones

Software y Firmware

QuantiFluor® Dye Systems Data Analysis Workbook

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QuantiFluor® ONE dsDNA System

Fluorescent Quantitation of dsDNA with "Add-and-Read" Convenience

Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

DNA Quantitation Assay Sensitivity and Linearity

Lambda DNA

Easy Setup on Microplate or Single-Tube Fluorometers