QuantiFluor® RNA System

The QuantiFluor® RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is  important for many biological applications, including determining yield of in vitro transcribed RNA and  measuring RNA concentration before Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, RT-PCR and differential display PCR.

The QuantiFluor® System requires less RNA (100pg) than other RNA quantitation methods (~1µg), so you save more of your sample for downstream assays.

The QuantiFluor® RNA System detects as little as 0.5ng/ml RNA in a 96-well microplate assay (corresponding to 1µl of a 100ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.