RNasin® Plus Ribonuclease Inhibitor
RNase Protection with Enhanced Heat and Oxidation Resistance
- Maintains RNase inhibitory activity over a wide temperature range
- Inhibits a broad spectrum of eukaryotic RNases over a wide pH range (pH 5–8)
- Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™ Reverse Transcriptase, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase
- Animal origin-free (AOF) and cGMP-manufactured version available for RNA therapeutic applications. Learn more.
Catalog Number:
Choose a concentration
Size
RNasin®: The Most Trusted Name in RNase Inhibition
Promega RNasin® Ribonuclease Inhibitors are the gold standard for RNase protection. RNasin inhibitors feature in many publications and are the most widely used protection against RNases worldwide. Use RNasin® Ribonuclease Inhibitors for RNA isolation, RT-PCR, in vitro transcription or any situation where RNase contamination is a concern.
RNasin® Plus RNase Inhibitor inhibits ribonucleases during reaction assembly. Heating of reactions to as high as 70°C for 15 minutes does not result in reappearance of ribonuclease activity, thus RNasin® Plus can be added prior to RT-PCR to offer enhanced protection of RNA during the reverse transcription reaction.
Features of RNasin® Plus Ribonuclease Inhibitor:
- Inhibits RNase A, B and C as well as human placental RNase.
- Does not inhibit other RNases and modifying enzymes like reverse transcriptases.
- Results in higher yield in RNA extractions and better performance in RT-PCR, cDNA synthesis, microarrays and in vitro transcription/translation
- Stable against oxidation and heat, leading to higher stability—up to 15 minutes at 70 °C.
- Particularly suitable for long-term storage of samples.
RNasin® Plus is Stable at Elevated Temperatures
RNasin® Plus RNase Inhibitor is a recombinant mammalian RNase inhibitor capable of inhibiting eukaryotic RNases, such as RNase A and RNase B, similar to the human version of the protein. Certain cysteine residues present in human placental RNase inhibitor have been implicated in the oxidation sensitivity of that enzyme (1). RNasin® Plus RNase Inhibitor does not contain these cysteine residues, which makes it more resistant to oxidative damage. RNasin® Plus RNase Inhibitor forms a stable complex with RNases, inactivating the RNase. This complex is stable at temperatures up to 70°C for at least 15 minutes (Figure 1).
RNasin® Plus has been tested in RT-PCR and, like the human protein, is compatible with enzymes like AMV, M-MLV or ImProm-II™ Reverse Transcriptases as well as Taq and Tfl DNA Polymerases. RNasin® Plus has also been tested and found to be compatible with quantitative real-time RT-PCR in a TaqMan® Assay, and can even preserve templates from degradation in a biological extract as complex as a rat liver protein extract, demonstrating a broad spectrum of RNase inhibition (2).
- Kim, B.M., et al. R.T. (1999) Variants of ribonuclease inhibitor that resist oxidation. Protein Sci. 8, 430–4.
- Andrews, C. et al. (2003) RNasin® Plus RNase Inhibitor: New Protein for High Temperature RNase Inhibition. Promega Corporation.
RNasin® Plus Source, Unit Definition, QC and Storage Buffer
Source
Recombinant E. coli.
Unit Definition
One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.
Storage Buffer
20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.
QC Assays
SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
RNasin® Plus RNase Inhibitor |
N261A | 1 × 2,500u | 40u/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
RNasin® Plus RNase Inhibitor |
N261B | 1 × 10,000u | 40u/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Resources
Related Products
Usado con frecuencia junto con
ReliaPrep™ miRNA Cell and Tissue Miniprep System
Purifica miARN de forma rápida y sin fenol, a partir de muestras de células de pequeño volumen y tejidos.
Z6210, Z6211, Z6212
ReliaPrep™ RNA Miniprep Systems
Extracción de ARN total a partir de 100 células o 0,25mg de tejido, con un sencillo protocolo de tan solo 30 minutos.
Z6010, Z6011, Z6012, Z6110, Z6111, Z6112
SV 96 Total RNA Isolation System
Purifica ARN total a partir de 96 muestras en menos de una hora. Formato basado en el vacío.
Z3500, Z3505
