ProNex® DNA QC Assay ABI 7500/7500FAST

Qualify and Quantify Your Samples in a Single Reaction

The ProNex® DNA QC Assay evaluates the quality and quantity of genomic DNA extracted from formalin-fixed paraffin-embedded (FFPE) samples or other potentially degraded DNA sources. It is a human-specific, multiplexed probe-based quantitative polymerase chain reaction (qPCR) assay that may also be used to evaluate the ratio of circulating cell-free DNA (ccfDNA) to higher molecular weight genomic DNA in plasma samples. The multiplex assay detects 75bp, 150bp and 300bp human genomic DNA sequences, and it includes an internal positive control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors.

One Step Toward a Better NGS Workflow

Using the ProNex® DNA QC Assay to determine the amplifiability and quantity of your precious sample can save you time and money.

Comparison of Quantitation and Quality Control Features

Not all QC analysis is the same. Only the ProNex® DNA QC Assay includes everything you need, with minimal sample usage.

Downstream Predictive Value for Degraded Samples

Due to the inherent variability of FFPE DNA quality, knowing the quantity of DNA alone is not reliably predictive of downstream assay success. The ProNex® DNA QC Assay allows you to determine the amount of amplifiable DNA in a sample. You can use the ProNex® DNA QC Assay to evaluate if a sample is suitable for downstream analysis using techniques such as next-generation sequencing (NGS) or droplet digital PCR (ddPCR).

14502MA-W — Four separate FFPE tumor tissue samples of varying age and fixation times from 4 different tissue types (breast, lung, colon and rectal) were purified with three different methods for a total of 31 extractions. Each DNA Sample was quantified using a single-target qPCR assay and normalized for 10ng input into the Swift Biosciences Accel-Amplicon™ 56G Oncology Panel. After sequencing, samples were retroactively processed with the ProNex® DNA QC Assay and a degradation ratio (75/300bp quantitation ratio) was calculated. Samples with high degradation ratios were clearly correlated with poor coverage uniformity of sequencing, enabling users of the DNA QC Assay to predict downstream performance.

Evaluate ccfDNA Samples for gDNA Contamination

Genomic DNA contamination of ccfDNA can mask low level mutations. You can use the ProNex® DNA QC Assay to measure gDNA contamination in ccfDNA, allowing you to assess sample quality and suitability for downstream processing.

14503MA-W — ccfDNA was extracted from a serum sample. DNA was quantified with the ProNex® DNA QC Assay, and the ratio of 75:300bp concentrations was measured. Low ratios (close to 1) indicate gDNA contamination, whereas ratios >10 indicate extremely clean ccfDNA. “Contaminated” indicates sample as extracted without cleanup. “Clean” sample was processed with the ProNex® Size-Selective DNA Purification system to perform a dual-sided size selection and removal of DNA greater than 300-400bp. The increase in 75:300bp ratio is a result of the removal of gDNA.

14504MA-W — To verify correlation of 75:300bp ratios generated by the ProNex® DNA QC Assay, samples shown in figure were run on the Agilent 2200 TapeStation System. The clean sample trace (teal) is equivalent to a high ratio on the ProNex® DNA QC Assay, whereas the contaminated sample trace (bronze) equates to a low ratio.

Process Your Data with the ProNex® DNA QC Assay Analysis Software

The ProNex® DNA QC Assay Analysis Software will process qPCR data generated with the ProNex® DNA QC Assay Kits. The software includes calculations and methods to define values for the following:

Standard Curves (acceptable r² and slope values)
Sample quantity (based on the Standard Curves)
Sample quality (possible inhibition, contamination, or degradation)

The ProNex® DNA QC Assay Analysis Software can import results from the Applied Biosystems® 7500, 7500 Fast or QuantStudio™ 6 Flex Real-Time PCR Systems, and the BioRad CFX96 Touch™ Real-Time PCR Detection System.

Download Analysis Software

Protocolos

Complete Protocol

ProNex® DNA QC Assay for Use on the Applied Biosystems® 7500/7500 Fast Real-Time PCR Systems Technical ManualPDF (1737 KB) – English

ProNex® DNA QC Assay Analysis Software Technical ManualPDF (5269 KB) – English

ProNex® DNA QC Assay Calibration Kit, 7500 Technical ManualPDF (1396 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
ProNex® DNA QC Dilution Buffer	NG101A	2 × 1.5ml
2X ProNex® DNA QC Master Mix	NG121A	2 × 1.1ml
20X ProNex® DNA QC Primer-Probe-IPC Mix, ABI 7500/7500FAST	NG150A	1 × 220μl
ProNex® DNA QC gDNA Standard	NG302A	1 × 150μl	50ng/μl
Nuclease-Free Water	P119A	2 × 1,250μl		View Product

SDS

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Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Contenido

Item	Part #	Presentación	Concentración	Available Separately
ProNex® DNA QC Dilution Buffer	NG101A	8 × 1.5ml
2X ProNex® DNA QC Master Mix	NG121A	8 × 1.1ml
20X ProNex® DNA QC Primer-Probe-IPC Mix, ABI 7500/7500FAST	NG150A	4 × 220μl
ProNex® DNA QC gDNA Standard	NG302A	4 × 150μl	50ng/μl
Nuclease-Free Water	P119A	8 × 1,250μl		View Product

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Contenido

Item	Part #	Presentación
ProNex® DNA QC Calibration Standard, FAM	NG106A	1 × 60μl
ProNex® DNA QC Calibration Standard, CFG540	NG107A	1 × 60μl
ProNex® DNA QC Calibration Standard, TAMRA	NG108A	1 × 60μl
ProNex® DNA QC Calibration Standard, Q670	NG109A	1 × 60μl
ProNex® DNA QC Calibration Standard, CXR	NG110A	1 × 60μl
ProNex® DNA QC Calibration Buffer	NG111A	1 × 30ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Recursos

Artículos

Menciones

Development and Validation of Molecular Assays for Limited Tissue Samples.

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2019 Acta Cytol.
Ver todas las menciones

Posters

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ProNex® DNA QC Assay ABI 7500/7500FAST

Evaluate Quality and Quantity of gDNA from Degraded Samples

Qualify and Quantify Your Samples in a Single Reaction

One Step Toward a Better NGS Workflow

Comparison of Quantitation and Quality Control Features

Downstream Predictive Value for Degraded Samples

Evaluate ccfDNA Samples for gDNA Contamination

Process Your Data with the ProNex® DNA QC Assay Analysis Software

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Artículos

Menciones

Posters

ProNex® DNA QC Assay ABI 7500/7500FAST

Evaluate Quality and Quantity of gDNA from Degraded Samples

Qualify and Quantify Your Samples in a Single Reaction

One Step Toward a Better NGS Workflow

Comparison of Quantitation and Quality Control Features

Downstream Predictive Value for Degraded Samples

Evaluate ccfDNA Samples for gDNA Contamination

Process Your Data with the ProNex® DNA QC Assay Analysis Software

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Artículos

Menciones

Posters

Productos Similares

ProNex® DNA QC Assay BioRad CFX96™

ProNex® NGS Library Quant Kit

QuantiFluor® ONE dsDNA Dye

QuantiFluor® ONE dsDNA System

Usado con frecuencia junto con

ProNex® Size-Selective Purification System

ReliaPrep™ FFPE gDNA Miniprep System

Maxwell® RSC DNA FFPE Kit

Maxwell® RSC ccfDNA Plasma Kit

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