ProDye® Terminator Sequencing System

Sanger Sequencing in Perfect Harmony with Your Workflow

The ProDye® Terminator Sequencing System provides an improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix. Compatible with a variety of DNA templates, the ProDye® Terminator Sequencing System can be used with the Spectrum Compact CE System as well as the Applied Biosystems® 3130, 3130xl, 3500, 3500xL, 3730, 3730xl and SeqStudio® Genetic Analyzers. The ProDye® Terminator Sequencing System uses dTMR, dCXR, dRSixG and dROneTen terminator dyes, the same dyes contained in the BigDye® Terminator V3.1 Cycle Sequencing Kit.

Your Sample, Our Sequencing Kit, Any Instrument

Integrate the ProDye® Terminator Sequencing System seamlessly into a familiar workflow.

Dye-terminator sequencing workflow illustration.

Familiar Reaction Setup

Includes all the components for the sequencing reaction in a ready-to-use, premixed format—just add your template and the template-specific primer.

Proprietary enzyme blend in 2.5X Master Mix
Additional 5X Sequencing Buffer and Nuclease-Free Water is included
Supports 10μl reactions and 20μl 0.5X reactions

ProDye includes the dye-terminator sequencing reaction components in a premixed format

Compatible with a Variety of Thermal Cyclers

Simple cycling protocol designed for obtaining maximum read lengths.

Compatible with a Variety of Samples and Running Conditions

Sequence plasmid DNA and other samples using 'Standard' or 'Fast' sequencing protocols.

Example data using the ProDye™ Terminator Sequencing System to sequence plasmid DNA using the standard protocol. — **Chromatograms from NIST plasmid ERCC-00083 cycle sequenced with ProDye® Terminator Sequencing System and detected on the Spectrum Compact CE System using the ‘Standard’ sequencing protocol.** Plasmid ERCC00083 from NIST was cycle sequenced with ProDye® Terminator Sequencing System. Sequencing reactions were purified with ethanol/EDTA-based precipitation and denatured in Hi-Di™ Formamide before detection on the Spectrum Compact CE System using the T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B).

Sequencing Analysis Read Lengths

Combine ProDye® Terminator Sequencing System with the Spectrum Compact CE System for more bases in less time.

Example data showing sequencing reads of 600 bases and 800 bases. — Chromatograms from control pGEM®-3Zf(+) plasmid cycle sequenced with ProDye® Terminator Sequencing System and detected on the Spectrum Compact CE System using T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B).

Product Application: Sanger Sequencing of SARS-CoV-2

Sequence a region of the SARS-CoV-2 genome using the ProDye® Terminator Sequencing System with analysis by capillary electrophoresis on the Spectrum Compact CE System.

Download Product Application

Learn about the Spectrum Compact CE System

ProView® Sequencing Software

ProView® Sequencing Software is a customizable Sanger sequencing viewer capable of displaying .ab1 files generated on many currently available capillary electrophoresis (CE) sequencers. This software can be used to view and edit sequence information, display electropherograms, generate reports, display embedded information and save edited files in multiple formats.

Get ProView® Sequencing Software for Free

Example Proview sequencing software data

Comprehensive Technical Support

Technical support scientists are available to answer your questions

We understand that your work can’t stop and we are dedicated to minimizing interruptions to your lab workflow. Whether you are working with a new sample type, implementing Sanger sequencing on a new instrument, or anything in between, you can be confident that your sequencing will hit all the right notes with our comprehensive technical support. Promega scientists are ready to help, and we will be there when you need us.

Explore Support Options

Protocolos

Complete Protocol

ProDye™ Terminator Sequencing System Technical ManualPDF (11023 KB) – English

Contenido

Item	Part #	Presentación
ProDye® 2.5X Master Mix	CR100A	1 × 200μl
ProDye® 5X Sequencing Buffer	CR101A	1 × 1ml
pGEM®-3Zf(+) Vector	P227A	1 × 20μg
pUC/M13 Primer, Forward (24mer)	Q560A	1 × 2μg
Nuclease-Free Water	P119A-C	1 × 1.25ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Contenido

Item	Part #	Presentación	Available Separately
ProDye® 2.5X Master Mix	CR100B	2 × 800μl
ProDye® 5X Sequencing Buffer	CR101A	3 × 1ml
pGEM®-3Zf(+) Vector	P227A	1 × 20μg
pUC/M13 Primer, Forward (24mer)	Q560A	1 × 2μg
Nuclease-Free Water	P119A	5 × 1,250μl	View Product

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Contenido

Item	Part #	Presentación	Available Separately
ProDye® 2.5X Master Mix	CR100B	10 × 800μl
ProDye® 5X Sequencing Buffer	CR101B	1 × 12ml
pGEM®-3Zf(+) Vector	P227A	1 × 20μg
pUC/M13 Primer, Forward (24mer)	Q560A	1 × 2μg
Nuclease-Free Water	P119E	2 × 13ml	View Product

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

ProDye® Terminator Sequencing System

Sanger Sequencing Chemistry for Capillary Electrophoresis Platforms

Sanger Sequencing in Perfect Harmony with Your Workflow

Your Sample, Our Sequencing Kit, Any Instrument

Familiar Reaction Setup

Compatible with a Variety of Thermal Cyclers

Compatible with a Variety of Samples and Running Conditions

Sequencing Analysis Read Lengths

Product Application: Sanger Sequencing of SARS-CoV-2

ProView® Sequencing Software

Comprehensive Technical Support

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Recursos

Artículos

Otros

ProDye® Terminator Sequencing System

Sanger Sequencing Chemistry for Capillary Electrophoresis Platforms

Sanger Sequencing in Perfect Harmony with Your Workflow

Your Sample, Our Sequencing Kit, Any Instrument

Familiar Reaction Setup

Compatible with a Variety of Thermal Cyclers

Compatible with a Variety of Samples and Running Conditions

Sequencing Analysis Read Lengths

Product Application: Sanger Sequencing of SARS-CoV-2

ProView® Sequencing Software

Comprehensive Technical Support

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Recursos

Artículos

Otros

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Spectrum Compact CE System

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