HaloPROTAC3 Technical Manual
Instructions for Use of Product(s)
GA3110, GA4110
Literature # TM642
HaloPROTAC3 is a small-molecule degrader that specifically binds to and degrades the HaloTag® protein and its fusion partners in live cells. HaloPROTAC3 binds irreversibly to the HaloTag® protein and HaloTag®-target fusions, recruiting them via co-engagement with von Hippel Lindau (VHL), an E3 ligase component, to active E2/E3 ubiquitin ligase complexes. As a result, the HaloTag® protein and HaloTag®-target fusions are ubiquitinated and subsequently degraded by the proteasome. This approach of using a fusion tag PROTAC to degrade specific targets means broad study of degradation of numerous targets is possible, including phenotypic study of loss of specific isoforms or mutations, without the need for target-specific PROTACs. Furthermore, targeted protein degradation studies can be performed even if no target-specific PROTACs are available.
Created 1/21.