HaloLink™ Resin for Protein Pull-Down and Analysis

Marjeta Urh, Dan Simpson, Jacqui Sankbeil, Danette Hartzell, Natasha Karassina, Nadine Nassif, Jami English, Ji Zhu, Poncho Meisenheimer, Dieter Klaubert, Bob Bulliet, and Ketih V. Wood
Promega Corporation and Promega Biosciences, Inc.
Publication Date: 2006

Abstract

HaloLink™ Resin provides a method for specific, covalent and oriented immobilization of proteins onto surfaces. Here we demonstrate the high binding capacity and minimal nonspecific binding of the HaloLink™ Resin. We also show that the covalent HaloTag® bond provides stability during dilution and stringent washing, minimizing the loss of the HaloTag® fusion proteins from the surface. By analyzing protein:protein interactions and enzyme activity for several fusion proteins, we show that HaloTag® fusion proteins bound to the HaloLink™; Resin maintain function. HaloLink™ Resin is not a purification resin; however, in conjunction with protease cleavage, it can be used to isolate a protein of interest.

Cell Notes 14, 15–19.