Purifying His-Tagged Proteins from Insect and Mammalian Cells

Natalie Betz
Promega Corporation
Publication Date: 2004

Abstract

The FastBreak™ Cell Lysis Reagent, 10X and MagneHis™ Protein Purification System can be used to purify His-tagged proteins from insect and mammalian cell lysates and culture media in the presence or absence of serum. This article describes methods for using these reagents with insect and mammalian cells.

Cell Notes 9, 6–9.