A Polyclonal HaloTag® Antibody for Western Blotting and Immunocytochemistry

Randall D. Learish
Promega Corporation
Publication Date: 2007

Abstract

Here we describe a polyclonal antibody generated against a purified HaloTag® protein. Ligand binding does not significantly interfere with antibody binding epitopes and thus allows co-labeling with HaloTag® Ligands. The Anti-HaloTag® pAb can be used in traditional Western blotting applications with conjugated anti-rabbit secondary antibodies for colorimetric or chemiluminescent detection or immunocytochemistry.

Promega Notes 95, 20–22.