Transfection Quality Plasmid DNA in as Little as Ten Minutes Using the PureYield Plasmid Miniprep System

Don Smith and Eric Vincent
Promega Corporation
Publication Date: 2008

Abstract

Scientists require straightforward methods for plasmid purification as the first step in many laboratory protocols. Here we introduce the new PureYield™ Plasmid Miniprep System, which allows researchers to isolate high-quality plasmid DNA suitable for a broad range of downstream applications, including transfection, in vitro expression, sequencing and cloning. The system allows direct purification of plasmid from as much as 0.6 ml of bacterial culture without cell harvesting or from up to 3 ml of culture when the cells are pelleted. The entire procedure can be completed in as little as 10 minutes.

Promega Notes 100, 6–8.