[U-13C, 15N] Protein Labeling Using Escherichia coli Strain KRX
Kate Qin Zhao, Marco Tonelli, Jim Hartnett, and Michael Slater
1Promega Corporation, 2NMRFAM, University of Wisconsin-Madison
Publication Date: 2008
1Promega Corporation, 2NMRFAM, University of Wisconsin-Madison
Publication Date: 2008
Abstract
We have developed a protocol for efficient [U-13C, 15N]labeled protein production in the highly competent E. coli strain, KRX, using Silantes CN OD2 medium. This procedure can facilitate protein analysis by NMR spectroscopy and other methodologies that require 13C and 15N protein labeling.
Promega Notes 98, 6–7.
Learn about competent cells designed for tightly controlled protein expression.
