The Role of Cell-Free Rabbit Reticulocyte Expression Systems in Functional Genomics

Abstract

In the broad area of functional genomics, that is the global characterization of proteins and their function, cell-free rabbit reticulocyte lysate (RRL) has been used extensively to elucidate the mechanisms of mammalian translation, cotranslational modifications, post-translational modifications and translocation of proteins. More recently, RRL has been used as the workhorse for manufacturing  the proteins engaged in interaction, selection and protein evolution studies from DNA or mRNA libraries either in microarray, display or in vitro expression cloning (IVEC) technologies. This chapter highlights recent functional proteomics applications that use cell-free mammalian RRL.

Article

Related Products

• Expresión de proteínas sin células

Related Protocols

• Flexi® Rabbit Reticulocyte Lysate Systems Technical Bulletin
• TNT® Coupled Wheat Germ Extract Systems Technical Bulletin
• Rabbit Reticulocyte Lysate Technical Manual
• TnT® Quick Coupled Transcription/Translation System Technical Manual
• TNT® SP6 High-Yield Wheat Germ Protein Expression System Technical Manual
• TNT® T7 Insect Cell Extract System Protocol
• TNT® T7 Quick for PCR DNA Technical Manual

Related Resources

• Protein Purification
• Protein Expression

Frequently Asked Questions

• Can I detect a protein expressed in the TnT® Coupled in vitro Transcription/Translation System using Coomassie® blue-stained SDS-PAGE?

Related Articles

• Selecting the Cell-Free Protein Expression System That Meets Your Experimental Goals
• All PubHub Articles »