Isolation of Purified DNA Using a Bone DNA Extraction Kit
Part # PS339
Abstract
Lotte Downey1, Arune Brekke1, Douglas Horejsh1, Marjeta Urh1, Venus Kallupurackal2 and Anupama Gopalakrishnan1
1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
2Zurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland
We assessed a cellulose based resin in comparison with DNA IQ™ resin and bench-marked against a modified organic method for processing contemporary and old bones. Additionally, preprocessing buffers – Bone Incubation Buffer and Demineralization Buffer (Lorielle et al., 2007 ) were compared for DNA extraction efficiency using the DNA IQ™ resin chemistry and further compared to a competitor workflow. The results indicate that preprocessing of bone samples using demineralization buffer, followed by DNA purification using the DNA IQ™ chemistry offers an easy, automatable workflow for obtaining DNA that is pure and free of inhibitors. The extracted DNA was compatible with downstream applications such as PowerQuant® System and PowerPlex® Systems, thus enabling accurate human DNA quantification and STR profile generation.
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