Microsatellite Instability Analysis and NGS with Fragmented Sample Types

Part # PS324

Abstract

Samantha R. Lewis, Charles Cowles, Kathryn Oostdik, Curtis Knox, Brad Hook
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

A significant hurdle to using fragmented DNA for genomic studies is obtaining a sample of sufficient quantity and quality for rigorous downstream applications like NGS. Having effective tools to isolate, characterize, and analyze fragmented DNA containing samples, such as circulating cell free DNA (ccfDNA) and FFPE tissues, can prevent downstream failures, ultimately saving hours of work and precious samples. Here we present optimized methods for use with even highly fragmented DNA samples. Using this toolset, we demonstrate successful NGS and microsatellite instability (MSI) workflows using matched FFPE tissues and plasma samples.

Printed in USA.