Y-Screening and Direct Amplification of Sexual Assault Samples

Part # PS294

Abstract

Jeanne M. Bourdeau¹, Robert S. McLaren1¹, Amy B. McGuckian², Jerome G. Remm², Julie Conover Sikorsky², and Douglas R. Storts¹.
¹Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711; ²Palm Beach County Sheriff’s Office, West Palm Beach, FL, 33406

Screening for sperm in sexual assault samples is a laborious process with limited sensitivity in comparison to male DNA detection by amplification. Direct amplification of casework samples reduces both cost (labor & reagent) as well as the for potential DNA loss during purification, a special concern with low template samples. The prototype Casework Direct Solution Kit rapidly generates lysates from casework samples that may be amplified with the PowerQuant®System to screen sexual assault samples for male DNA and to normalize human template for STR amplification with one of the PowerPlex®Systems.

Printed in USA.