CloneWeaver® Workflow Purchasing Tool

5M Sodium Chloride is commonly used in many molecular biology and forensic applications.

Acrylamide, Molecular Grade, is used for the electrophoretic separation of nucleic acids and proteins. Very small DNA fragments, such as those generated by sequencing reactions, can be resolved by polyacrylamide gel electrophoresis. Proteins can be separated by a variety of techniques, including denaturing gel electrophoresis using SDS or urea, isoelectric focusing and native gel electrophoresis in a wide variety of buffers.

Agarose, LE, Analytical Grade, is used for the electrophoretic separation of nucleic acids.

Agarose, LMP, Preparative Grade for Large Fragments (>1,000bp), is a premium agarose used for isolating DNA fragments larger than 1,000bp. Each lot is tested and certified for the following applications: 1) restriction digestion, 2) ligation and transformation, and 3) random prime labeling. LMP = low melting point (i.e., Visit Product Page »

Agarose, LMP, Preparative Grade for Small Fragments (10 to 1,000bp), is a premium agarose used for isolating DNA fragments from 10 to 1,000bp. The isolated DNA fragments can be used in various molecular biology applications: 1) restriction digestion, 2) ligation and transformation, and 3) random prime labeling. LMP = low melting point (i.e., Visit Product Page »

Agarose, Low Melting Point, Analytical Grade, is ideal for applications that require recovery of intact DNA fragments after gel electrophoresis.

Antibiotic G-418 Sulfate is an aminoglycosidic antibiotic toxic to both prokaryotic and eukaryotic cells. It acts by interfering with protein synthesis and is used as an agent for selection of cultured cells expressing a gene (i.e., aminoglycoside 3' phosphotransferase [APH 3']) that confers resistance to G-418. The liquid form of the product is in distilled water and aseptically filtered. Formula Weight: 692.6 (anhydrous). Form: White powder. Physical/Chemical Properties of Powder: Appearance: White powder. TLC: Single major spot. Elemental Analysis: %C = 28.8–36.07; %H = 5.76–7.76; %N = 6.72–8.41. Absorbance: A280 (1mg/ml) = 0–0.015; A570 (100mg/ml) = 0–0.1. Specific Rotation: +104 to +121. Properties Specific to V7983: Appearance: White powder. Hydration Waters: 0–6, as determined from Elemental Analysis. Potency: >500μg/mg. Properties Specific to V8091: Potency: 40–60mg/ml. Sterility: Aseptically filtered.

Bisacrylamide, Molecular Grade, is a cross-linking agent used in the preparation of polyacrylamide gels. This product is tested for its efficiency in gel polymerization.

Blue/Orange Loading Dye, 6X, is a convenient marker dye containing 0.4% orange G, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 15% Ficoll 400, 10mM Tris-HCl (pH 7.5) and 50mM EDTA (pH 8.0). It is provided in a premixed, ready-to-use form. The dye is used for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis. In a 0.5-1.4% agarose gel in 0.5X TBE, xylene cyanol FF migrates at approximately 4kb, bromophenol blue at approximately 300bp and orange G at approximately 50bp.

Boric Acid, Molecular Biology Grade, in conjunction with Tris, is commonly used in buffers for the preparation of agarose or acrylamide gels and their associated running buffers.

Ethidium Bromide Solution, Molecular Grade (10mg/ml), is a fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. The solution can be used to detect both double-stranded and single-stranded DNA.

Guanidine Thiocyanate, Molecular Grade, at high concentrations, is a protein denaturant used most commonly for the isolation of intact RNA due to its ability to inhibit RNase.

Guanidine-HCl, Molecular Grade, is commonly used for the isolation of intact mRNA from tissues or cultured cells.

IPTG, Dioxane-Free (isopropyl-beta-D-thiogalactopyranoside), is an inducer of beta-galactosidase activity in many bacteria. Functioning as a lac analog, IPTG induces beta-galactosidase activity by binding to and inhibiting the lac repressor. This product is used to differentiate recombinants from nonrecombinants in cloning strategies using vectors containing the lacZ or lacZ alpha-peptide gene.

Urea is a protein denaturant. Urea is qualified for use as the denaturing component in polyacrylamide gels.

X-Gal, in conjunction with IPTG, is used to detect beta-galactosidase activity to differentiate recombinants from nonrecombinants in cloning experiments using vectors containing the lacZ or lacZ alpha-peptide gene.