qPCR Data Selector | Real-time qPCR Data | qPCR Performance Data

Real-Time qPCR Data Selector

In this selector, you can view experimental qPCR data generated with GoTaq® qPCR and RT-qPCR Systems using different instruments, templates, sample types, and more. Use the filters below to select your options and see the data.

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Target

Chemistry

Template

DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on an ABI 7500 instrument. Equivalent Cq values were observed for RNaseP and APP regardless of cycling conditions used for the experiment.

Commercially available human K562 genomic DNA (Cat.#E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP. The three genes gave equivalent Cq values regardless of cycling conditions used for the experiment.

DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on an ABI 7500 instrument. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq®1-Step RT-qPCR System (Cat.# A6020) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.

Commercially available human K562 genomic DNA (Cat.#E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on the Bio-Rad CFX96 qPCR instrument. The three genes gave equivalent Cq values regardless of the thermalcycling conditions used for the experiment.

DNA (10ng) from a lung tissue FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.

DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.

Commercially available human K562 genomic DNA (Cat.#E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on the Bio-Rad CFX96 qPCR instrument. The three genes gave equivalent Cq values regardless of the thermalcycling conditions used for the experiment.

DNA (10ng) from a lung tissue FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a BioRad CFX96 instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a BioRad CFX96 instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a BioRad CFX96 instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.

Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 96 well plate. The three genes have equivalent Cq values regardless of the thermalcycling conditions used for the experiment.

DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. RNaseP and APP had equivalent Cq values regardless of cycling conditions used for the experiment.

Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 96 well plate. The three genes have equivalent Cq values regardless of the thermalcycling conditions used for the experiment.

DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 96 well plate instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 96 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 instrument with a 96 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for RNaseP and APP regardless of cycling conditions used for the experiment.

Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 384 well plate. RNaseP and APP gave equivalent Cq values regardless of the thermalcycling conditions used for the experiment.

DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument with a 384 well plate. Equivalent Cq values were observed for RNaseP and APP evaluated regardless of cycling conditions used for the experiment.

DNA (10ng) from whole blood was amplified using the GoTaq(R) Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for APP regardless of cycling conditions used for the experiment.

Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 384 well plate. APP samples showed equivalent Cq values regardless of the thermalcycling conditions used for the experiment.

DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Lightcycler 480 instrument with a 384 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for B2M and HPRT1 evaluated regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 384 well plate using both standard and Fast thermalcycling conditions.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 384 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument looking at RNaseP, TERT and APP.

Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on the Stratagene Mx3005P qPCR instrument.

DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument looking at RNaseP, TERT and APP.

DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument looking at RNaseP and APP.

Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on the Stratagene Mx3005P qPCR instrument.

DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument. Equivalent Cq values were observed for RNaseP and APP regardless of cycling conditions used for the experiment.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on a Stratagene Mx3005P using both standard and Fast thermalcycling conditions.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on a Stratagene Mx3005P using both standard and Fast thermalcycling conditions.

Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq(R) 1-Step RT-qPCR System (Cat.# A6020) on a Stratagene Mx3005P using both standard and Fast thermalcycling conditions.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) looking at a high expressed gene, GNB2L1.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) looking at a low expressed gene, HPRT1.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) looking at a moderately expressed gene, B2M.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated by looking at the gene expression levels of three genes (GNB2L1, HPRT1, and B2M) using the GoTaq(R) Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq(R) System was able to detect expression levels all three genes from RNA purified from two different microtissue densities.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020) looking at a high expressed gene, GNB2L1.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020) looking at a low expressed gene, HPRT1.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020) looking at a high expressed gene, B2M.

HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated by looking at the gene expression levels of three genes (GNB2L1, HPRT1, and B2M) using the GoTaq(R) 1-Step RT-qPCR System (Cat. # A6020). The GoTaq(R) System was able to detect all three genes from RNA purified from two different microtissue densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101) looking at the APP gene. The GoTaq(R) Probe Master Mix was able to detect APP from three different microtissue cell densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101) looking at the TERT gene. The GoTaq(R) Probe Master Mix was able to detect TERT from three different microtissue cell densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101) looking at the RNaseP gene. The GoTaq(R) Probe Master Mix was able to detect RNaseP from three different microtissue cell densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101). The GoTaq(R) Probe Master Mix was able to detect APP, TERT, and RNaseP from gDNA purified from three different microtissue densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) qPCR Master Mix (Cat. # A6001) looking at the APP gene. The GoTaq(R) Master Mix was able to detect APP from three different microtissue cell densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) qPCR Master Mix (Cat. # A6001) looking at the TERT gene. The GoTaq(R) Master Mix was able to detect TERT from three different microtissue cell densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) qPCR Master Mix (Cat. # A6001) looking at the RNaseP gene. The GoTaq(R) Master Mix was able to detect RNaseP from three different microtissue cell densities.

HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq® qPCR Master Mix (Cat. # A6001). The GoTaq® Master Mix was able to detect APP, TERT, and RNaseP from gDNA purified from three different microtissue densities.

Circulating Cell Free DNA (CCF DNA) was purified from plasma using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) Probe qPCR Master Mix was able to amplify all three genes from purified ccfDNA.

Circulating Cell Free DNA (CCF DNA) was purified from serum using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) Probe qPCR Master Mix was able to amplify all three genes from purified ccfDNA.

Circulating Cell Free DNA (CCF DNA) was purified from plasma using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® qPCR Master Mix (Cat. # A6001) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) qPCR Master Mix was able to amplify all three genes from purified ccfDNA.

Circulating Cell Free DNA (CCF DNA) was purified from serum using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® qPCR Master Mix (Cat. # A6001) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) qPCR Master Mix was able to amplify all three genes from purified ccfDNA.

Four, tenfold dilutions of corn DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at the cl3036_1 LOC100192652 gene.

Four, tenfold dilutions of soybean DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at the ACPD gene.

Four, tenfold dilutions of Arabidopsis DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at the AT302610 gene.

Expression levels for the EF1a gene in corn were evaluated via 1-step RT-qPCR using the GoTaq® 1-step RT-qPCR System (Cat. # A6020).

Expression levels for the Cons6 gene in soybean were evaluated via 1-step RT-qPCR using the GoTaq® 1-step RT-qPCR System (Cat. # A6020).

Expression levels for the EF1A gene in Arabidopsis were evaluated via 1-step RT-qPCR using the GoTaq® 1-step RT-qPCR System (Cat. # A6020).

Expression levels for the Zmm19 gene in corn were evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120).

Expression levels for the PEPC17 gene in soybean were evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120).

Expression levels for the NY-YB1 gene in Arabidopsis were evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120).

Four, tenfold dilutions of corn DNA were amplified via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at the Zmm19 gene.

Four, tenfold dilutions of soybean DNA were amplified via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at the PEPC17 gene.

Expression levels for the GRAS gene in oil palm seeds were evaluated via 1-step RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020).

Expression levels for the GAPDH gene in coffee bean were evaluated via 1-step RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020).

Three, tenfold dilutions of oil palm seed DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) and universal plant primers targeting the ATP-1 gene.

Three, tenfold dilutions of rose seed DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) and universal plant primers targeting the ATP-1 gene.

Three, tenfold dilutions of coffee seed DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) and universal plant primers targeting the ATP-1 gene.

DNA purified from mouse lung tissue samples was analyzed via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.

DNA purified from mouse kidney tissue samples was analyzed via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.

DNA purified from mouse heart tissue samples was analyzed via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.

DNA purified from mouse lung tissue samples was analyzed via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® Probe qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.

DNA purified from mouse kidney tissue samples was analyzed via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® Probe qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.

DNA purified from mouse heart tissue samples was analyzed via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® Probe qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.

Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse lung tissue and the GoTaq® 1-Step RT-qPCR System (Cat. # A6020). The GoTaq® 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse lung tissue.

Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse kidney tissue and the GoTaq® 1-Step RT-qPCR System (Cat. # A6020). The GoTaq® 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse kidney tissue.

Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse heart tissue and the GoTaq® 1-Step RT-qPCR System (Cat. # A6020). The GoTaq® 1-Ste RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse heart tissue.

Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse lung tissue and the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq® Probe 1-Step RT-qPCR System was able to detect all three low expressed genes from mouse lung tissue.

Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse kidney tissue and the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq® Probe 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse kidney tissue.

Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse heart tissue and the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq® Probe 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse heart tissue.

Human plasma (300µl) was spiked with inactivated CMV particles, followed by DNA extraction using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit on the Maxwell® RSC instrument. CMV copies ranging from 10,000 to 100 were detected via qPCR using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Bio-Rad CFX96 instrument.

DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in singleplex (Panels A-C) and triplex (Panel D) reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.

DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in singleplex reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.

DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in singleplex reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.

DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in triplex reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.

Gene expression of B2M (VIC), HPRT (FAM), RNaseP (ABY), and GAPDH (JUN) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.

Gene expression of RNaseP (ABY) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.

Gene expression of B2M (VIC), HPRT (FAM), RNaseP (ABY), and GAPDH (JUN) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.

Gene expression of B2M (VIC), HPRT (FAM), RNaseP (ABY), and GAPDH (JUN) was analyzed from commercially available total human colon RNA in quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.

Gene expression of RNaseP (ABY), GAPDH (JUN), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from human colon FFPE blocks in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Gene expression of RNaseP (ABY), GAPDH (JUN), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from HCT116 cells in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Gene expression of B2M (VIC), HPRT (FAM), GAPDH (JUN) and ß-actin (Cy5) was analyzed from 20ng of RNA purified from human lung FFPE blocks in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Gene expression of B2M (VIC), HPRT (FAM), GAPDH (JUN) and ß-actin (Cy5) was analyzed from 20ng of RNA purified from HCT116 cells in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.

Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from commercially available total human genomic DNA and triplex.

Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from commercially available total human genomic DNA and triplex.

Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from commercially available total human genomic DNA using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.

Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from 20ng of DNA purified from human lung FFPE blocks in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.

Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from 20ng of DNA purified from human ccfDNA samples in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.

Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from 20ng of DNA purified from human genomic DNA in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.

Gene expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from human lung FFPE blocks in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on a Roche Lightcycler 480 instrument.

Gene expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from HCT116 cells in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on a Roche Lightcycler 480 instrument.

Gene expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of commercially available human colon RNA in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on a Roche Lightcycler 480 instrument.

Expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of DNA purified from human gDNA in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Roche Lightcycler 480 instrument.

Expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of DNA purified from ccfDNA in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Roche Lightcycler 480 instrument.

Expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of DNA purified from human lung FFPE blocks in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Roche Lightcycler 480 instrument.

The 16S rRNA gene found in E. coli was detected from ten-fold serial dilutions of E. coli DNA ranging from 37ng to 3.7×10^–3ng using the GoTaq® qPCR Master Mix.

The 16S rRNA gene found in S. aureus was detected from ten fold serial dilutions of S. aureus DNA ranging from 126ng to 0.0126ng using the GoTaq® qPCR Master Mix.

The 16S rRNA gene found in Salmonella enterica was detected from ten fold serial dilutions of Salmonella enterica DNA ranging from 500,000fg to 50fg using the GoTaq® qPCR Master Mix.

The stx1 gene found in E. coli was detected from ten fold serial dilutions of E. coli DNA ranging from 37ng to 3.7pg using the GoTaq® Probe qPCR Master Mix.

The nucA gene found in S. aureus was detected from ten fold serial dilutions of S. aureus DNA ranging from 126ng to 0.0126ng using the GoTaq® Probe qPCR Master Mix.

The ttr gene found in Salmonella enterica was detected from ten fold serial dilutions of Salmonella enterica DNA ranging from 500pg to 0.5pg using the GoTaq® Probe qPCR Master Mix.

The hly gene found in Listeria monocytogenes was detected from ten fold serial dilutions of Listeria monocytogenes DNA ranging from 500pg to 0.5pg using the GoTaq® Probe qPCR Master Mix.

A set of predetermined Armored HIV RNA copies were evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) in order to create a standard curve for HIV copy number determination in samples.

Cell culture medium was spiked with 12,500–50,000 copies of Armored HIV, followed by RNA extraction using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit on the Maxwell® RSC instrument. HIV RNA was detected via qPCR using the GoTaq® Probe 1- Step RT-qPCR System (Cat. # A6120) on a Bio-Rad CFX96 instrument.

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