New Small and Versatile Reporter Technologies for Challenging Applications in Virology
This webinar will demonstrate how NanoLuc® Luciferase:
- Could be inserted into size constrained viral genomes without attenuating the virus to any significant degree both in cell culture and animal models
- Was bright enough to enable in vivo imaging of viral infection, spread, clearance and immunity
- Allowed for the development of luminescent viral “titer” and cell binding assays that were very fast and sensitive
Summary
Luminescence-based assays are powerful research tools and integral components of many research projects including viral studies. However, in some cases the large size and limited brightness of the currently available luciferases limit their use in viral and other challenging applications. This webinar will introduce a new reporter, NanoLuc® Luciferase, which due to its extremely bright signal and small size, overcomes many of the challenges associated with the development of useful reporter viruses and assays. In addition, we’ll introduce a new luminescent binary reporter system, NanoBiT™, which enables a variety of investigations such as protein-protein interaction experiments previously not possible with single reporter protein systems. Specifically, this webinar will describe the NanoLuc® Reporter and NanoBiT™ Systems, three available NanoLuc® reporters (stable, destabilized and secreted), as well as provide data examples demonstrating the versatility and utility of these novel reporter systems for challenging viral applications. Information presented in this webinar should also be of value to researchers interested in applying these technologies to studies outside of virology.
Speaker
Robert Brazas, PhD
North America Marketing Manager
Rob has over 20 years of molecular biology bench experience and received his Ph.D. in Molecular Biology from the University of Utah where he studied the transcriptional regulation of the yeast HO gene using many techniques such as protein purification (native, his- and GST-tagged), gel shift assays, in vivo reporter assays, northerns, Southerns and other many other routine molecular biology techniques. He followed his Ph.D. with post-doctoral training in molecular virology at UCSF, and has held R&D positions in a few biotech companies where he worked on vaccine development and transfection reagent development. Rob has also contributed to epigenetic and cytogenetic microarray development and support before joining the Promega North American branch focusing on proteomics and genomics.