Tools to Improve Protein Sample Preparation for Mass Spec
This webinar will discuss common shortcomings of protein mass spec sample
preparation and provide solutions to these shortcomings.
Summary
Common shortcomings of protein mass spec sample preparation include incomplete digestion, lengthy and laborious procedure, low protein sequence coverage, inefficient peptide recovery of in-gel digested proteins or poor protein extraction from cells and tissues and inadequate instrument performance monitoring. In this webinar, we offer solutions to these shortcomings and discuss the advantages of trypsin preparations with enhanced proteolytic efficiency. We also review mass spec compatible SDS surfactant analogs and their advantage for in-gel protein digestion, peptide recovery and protein extraction from tissues. The discussion includes a case study showing positive impact of proteases with alternative cleavage specificity for increasing of sequence coverage of analyzed proteins. In the final part, we review the advantages of standardized total cell protein extracts and a mix of heavy isotope labeled peptide standards for optimization of protein mass spec sample preparation and monitoring of key performance parameters of mass spec instruments.
Speaker
Sergei Saveliev, PhD
Senior Research Scientist
Promega Corporation
Sergei Saveliev is a Senior Research Scientist at Promega. Prior to joining the industry Dr. Saveliev worked on various academic projects including epigenomics, DNA repair and recombination, enzymology, mechanisms of radioresistance and circadian rhythm. Dr. Saveliev’s research efforts were supported by US government funding, and the results were published in the leading academic journals such as the EMBO Journal, PNAS, PLOS, and Genes & Development. After joining Promega Corporation in 2004, Dr. Saveliev utilized his expertise to develop tools for life sciences. Lately, his product development efforts have been focused on tools for protein mass spectrometry sample preparation. Sergei has played the key role in building mass spectrometry protein analytical portfolio at Promega. His efforts are directed toward the development of solutions that help overcome address the major challenges in needs of protein mass spec sample analysis including efficient proteolysis, standardization, suppressing of side effects of protein mass spec sample preparation and others.