Tracking Cellular Protein Localization and Movement in Cells with a Flexible Fluorescent Labeling Technology

By knowing where a protein localizes in cells, researchers are one step closer to understanding a protein’s function. There are many methods for imaging proteins within cells including immunofluorescence-based assays and fluorescent protein tagging, but each of these methods limits the types of studies that can be performed. Here we describe the HaloTag® Technology, which is a small, universal protein tag engineered to covalently tether different functional groups to HaloTag® fusion proteins in living cells (or in vitro) such as, in the case of this webinar, a variety of fluorescent dyes. This technology enables researchers to easily perform many different types of protein imaging experiments including simple protein localization and the protein movement within a cell in real-time. This webinar will provide an overview of this unique technology including how it works and the various applications possible with HaloTag® with a focus on protein imaging. Then we will provide data illustrating these capabilities using both data generated in-house and in published, peer reviewed scientific publications.