HaloTag Figures/Sequences
Verification Data
Insert Size Confirmation
Insert size is confirmed by agarose gel electrophoresis.
In addition both the N-terminus and C-terminus are sequenced by single-pass sequencing.
Insert size confirmation in agarose gel for Clone: FHC01823, Lane: E05
Expression Validation
All pFN21A ORF clones are transiently expressed in HEK-293T
cells, after labeling the cell lysate with HaloTag TMR ligand the expression and
molecular mass is validated on a SDS PAGE gel.
Expression Validation in SDS-PAGE gel for Clone: FHC01823, Lane: 9
Cell Imaging Validation
Using fluorescent microscopy the sub-cellular localization
of the ORF is visualized after labeling with HaloTag TMR ligand.
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ORF Clone Information
Vector Restriction Map
Vector + ORF Sequence
Native (Untagged) Figures/Sequences
Verification Data
Protein expression and microscopic cell imaging is only performed on HaloTag® ORF
Clones.
ORF Clone Information
Vector Restriction Map
Vector + ORF Sequence