ΦX174, RF DNA

D1531_PhiX174--RF-DNA--50ug_3
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Double-Stranded Circular DNA Molecule Used to Detect Restriction Enzyme Assay Nickase Activity

  • Supplied at a concentration of 1mg/ml

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Catalog number selected: D1531

$ 129.00
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ΦX174, RF DNA
50μg
$ 129.00
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The icosahedral bacteriophage ΦX174 replicative form (RF) is a double-stranded circular DNA molecule of 5,386 bases. Restriction enzyme-digested ΦX174 DNA generates molecular weight size markers used in gel analysis of nucleic acids. ΦX174 DNA is often used in the assays of restriction enzymes for the presence of nickase activity.

Storage Buffer: 10mM Tris-HCl (pH 7.5), 1mM EDTA.

GenBank - EMBL Accession Number: NC001422.

Reference

  1. Sanger, F. et al. (1978) J. Mol. Biol. 125, 225–46.

Specifications

What's in the box?

Item Part # Size

ΦX174 RF DNA

 D153A 1 × 50μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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Restriction enzyme that cuts DNA at 37°C and leaves a 5´ overhang.

R6201, R6205

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