GenePrint® 24 System

24-Locus STR System for Mixed Sample Analysis with Abundant Source Material

The GenePrint® 24 System is designed to generate a 5-dye 24-multilocus human DNA profile from a variety of human-derived biological sources.

The number of loci allows for deconvolution of closely related samples with higher power of discrimination. Compatibility with databases around the world allows for fast identity confirmation and matching probability calculation, improving labs overall efficiency.

Relevant identification applications include:

Human cell line authentication and identification of closely related cells
Tracking and confirmation of tissue specimen provenance and cell lineage
Detection of contaminants, including extraneous tissue or mixed cell populations
Twin zygosity testing to determine whether twins are identical or fraternal
Fetal gender determination early in pregnancy
Databasing and biobanking

Configuration of the GenePrint® 24 System

Loci used in the GenePrint 24 System — The *GenePrint* 24 System allows co-amplification and five-color detection of 24 loci, D3S1358, D1S1656, D2S441, D10S1248, D13S317, D16S539, D18S51, D2S1338, CSF1PO, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, D22S1045, and FGA plus Penta E, Penta D, DYS391 and Amelogenin.

Improved Productivity, Better Detection of Low-Level Mixtures

GenePrint® 24 System is compatible with 2.5 to 5ng of extracted DNA samples with fewer PCR cycles in lower reaction volumes than previous STR systems. The small kit size (100 reactions) and low reaction volume (12.5µl) provides an economical benefit as well.

With the The GenePrint® 24 System you can:

Obtain optimal heterozygote balance using up to 5ng of DNA template
Achieve better consistency in detecting low level mixtures
Identify unique alleles to resolve complex mixtures from related individuals or multiple sources
Improve productivity with rapid-cycling and more loci
Simplify validation and continuity using loci in concordance with previously generated data
Implement a simple workflow matching your laboratory structure with protocols compatible for POP-4 and POP-7 in multiple CE instruments and capillary arrays
Use analysis methods for GeneMapper® and GeneMarker®

Higher Sample Input for Optimal Heterozygote Balance

Amplification of 2.5ng of human DNA using the GenePrint® 24 System analyzed with a Spectrum Compact CE System — Amplification of 2.5ng of human DNA using the *GenePrint*® 24 System analyzed with a Spectrum Compact CE System using a 1.6kV, 9-second injection.

Detect Mixtures and Cross-Contamination

Example electropherograms showing unique HeLa cell alleles detected in a cell mixture, using the GenePrint® 24 System and the Spectrum Compact CE System — Example TMR (yellow) dye channel electropherograms showing the unique HeLa cell alleles detected in a cell mixture, amplified with the *GenePrint*® 24 System and analyzed on the Spectrum Compact CE System. Outlined in blue is the locus D5S818.

Detection Instrument Compatibility and Data Analysis

The GenePrint® 24 System is compatible with the Spectrum Compact CE System and the Applied Biosystems® SeqStudio, 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

You may need to optimize protocols including the amount of template DNA, cycle number, injection conditions and loading volume for your laboratory instrumentation.

Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® and GeneMarker® software and are available for download.

Download Panels and Bins

Protocols

Complete Protocol

GenePrint® 24 SystemPDF (4398 KB) – English

GenePrint® 24 System for Use on the Spectrum Compact CE System Technical ManualPDF (14480 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
GenePrint® 24 5X Primer Pair Mix	B179A	1 × 250μl
GenePrint® 24 5X Master Mix	B185A	1 × 250μl
GenePrint® 24 Allelic Ladder Mix	B186A	1 × 50μl
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
WEN Internal Lane Standard 500	DG500A	1 × 200μl		View Product
Water, Amplification Grade	DW099A	3 × 1,250μl		View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-ET, CXR-ET and WEN dyes are proprietary.