G8090_Caspase-Glo--3-3a7-Assay--2-5ml_3 G8091_Caspase-Glo--3-3a7-Assay--10ml_3 G8093_Caspase-Glo--3-3a7-Assay--10-x-10ml_3 G8092_Caspase-Glo--3-3a7-Assay--100ml_3 mg1000-myglo-personal-reagent-reader

Caspase Activity Assay for Apoptosis Detection

  • High sensitivity caspase assay—requires fewer cells and less enzyme
  • Simple add-mix-measure protocol—no sample preparation needed
  • Easily scalable to 96-, 384- and 1,536-well plate formats
  • Integrates seamlessly with the MyGlo™ Reagent Reader

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$ 150.00

Catalog Number: G8090

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$ 470.00

Catalog Number: G8091

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$ 3,643.00

Catalog Number: G8093

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$ 2,677.00

Catalog Number: G8092

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$ 5,500.00

Catalog Number: MG1010

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Overview
Protocols
Specifications
Resources
Related Products
Citations

Homogeneous Assay Measures Caspase-3/7 Activity

The Caspase-Glo® 3/7 Assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by the luciferase, generating a "glow-type" luminescent signal that is proportional to caspase-3/7 activity.

The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference than fluorescent- or colorimetric-based assays. The caspase activity assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. The assay delivers excellent Z´-factor values in cell and purified enzyme models. Using the MyGlo™ Reagent Reader in conjunction with the ProNect™ Data Platform, you can visualize caspase activity with ease.

caspase 3 activity assay mechanism 9555ma-01

Need to measure caspase activity in 3D cultures? See our new Caspase-Glo® 3/7 3D Assay!

Learn More

Simple Bioluminescent Detection of Caspase Activity

Caspase 3 activity assay protocol 4059ma03-3b

Luminescence Is Linear Over a Broad Range of Cell Numbers

example caspase 3 assay results 4098MB-W

Jurkat cells were treated with anti-Fas mAb for 4.5 hours to induce apoptosis or were left untreated. Caspase-Glo® 3/7 Reagent was added directly to the cells in 96-well plates and incubated for 1 hour before recording luminescence. Each point represents the average of 4 wells. The "no cell" blank control value has been subtracted from each.


Measure Dose Response to Apoptosis Inducers

Dose-response graph showing an apoptotic response to bortezomib with no apoptotic response to palbociclib.

Jurkat cells were seeded at 10,000 cells/well and treated for 20 hours with two compounds. Caspase-Glo® 3/7 Reagent was added to the cells and luminescence measured on the MyGlo™ Reagent Reader. The dose-response graph shows an apoptotic response to bortezomib with no apoptotic response to palbociclib.


Multiplex Caspase 3/7 with Cytotoxicity and Cell Viability Assays

caspase-glo-3-7-multiplex-10651mb-w
Jurkat cells (10,000 cells/well) were treated with serially diluted staurosporine. Cell viability, cytotoxicity and apoptosis were assayed after 6 hours using the ApoTox-Glo™ Triplex Assay, featuring the Caspase-Glo® 3/7 Assay chemistry. Fold change from the untreated cells control (concentration = 0M) was calculated for all data points.

Notes

Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8090 provides sufficient reagents to perform 25 caspase activity assays; Cat.# G8091, 100 assays; Cat.# G8092 and G8093, 1,000 assays. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8090 provides sufficient reagents to perform 100 caspase activity assays; Cat.# G8091, 400 assays; Cat.# G8092 and G8093, 4,000 assays.

Specifications

Catalog Number:

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810C 1 × 2.5ml

Caspase-Glo® 3/7 Substrate

G811C 1 × 1 bottle

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810A 1 × 10ml

Caspase-Glo® 3/7 Substrate

G811A 1 × 1 bottle

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810A 10 × 10ml

Caspase-Glo® 3/7 Substrate

G811A 10 × 1 bottle

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810B 1 × 100ml

Caspase-Glo® 3/7 Substrate

G811B 1 × 1 bottle

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

What's in the box?

Item Part # Size Available Separately

MyGlo™ Reagent Reader

  • USB Cable
  • Wall Power Adapter
  • Plug Adapters
MG1000 1 × 1 each

CellTiter-Glo® 2.0 Reagent

G9241 1 × 10ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Citations

Recent publications that mention the use of this product.