Lumit® IFN-γ (Human) Immunoassay

w6040-lumit-ifn-y-human-immunoassay-100-assays
w6042-lumit-ifn-y-human-immunoassay-500-assays
w6041-lumit-ifn-y-human-immunoassay-1000-assays
w6040-lumit-ifn-y-human-immunoassay-100-assays
View information on global supply logistics
automation-support-promo

No-Wash Immunoassay for IFN-γ Detection

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a plate-reading luminometer
  • Scalable to 96- or 384-well plates

Size

Catalog number selected: W6040

$ 597.00
Your price:
Add to Cart
This product is discontinued
Request a Sample
Not available in all countries
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Lumit® IFN-γ (Human) Immunoassay
100 assays
$ 597.00
Your price: Log in
Change Configuration

Quantify Cytokine Release Without Tedious Wash Steps

The Lumit® IFN-γ Immunoassay quantitatively measures released IFN-γ in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® IFN-γ Immunoassay Works

Primary antibodies to IFN-γ were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IFN-γ, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IFN-γ levels.

How the Lumit™ IL-2 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Lumit™ IL-2 Immunoassay Workflow

Large Dynamic Range of Lumit® IFN-γ Detection

The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.

lumit ifn-gamma standard curve 17852ma

Assay

IFN-γ Human Immunoassay

Limit of Detection (LOD)

1.7pg/ml (3 SD above background)

Dynamic Range

7.3pg/ml – 10ng/ml

Minimal Detectable Dose (MDD)

1.1pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

Detect IFN-γ as a Marker of T Cell Activation

Data showing IFN-γ release as a Marker of T Cell Activation

Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). The Lumit® IFN-γ (Human) Immunoassay reagents were added directly to treated cell wells to detect IFN-γ release from the T cells.

Multiple Cytokine Detection by Split-Sample Analysis

Split-sample analysis for detection of IL-2, IL-6, IFN-γ and TNF-α using corresponding Lumit™ Cytokine Immunoassay.18001ma

Human PBMCs were plated in a 96-well plate in 100µl and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848. Four 20μl aliquots were transferred to a 384-well plate for detection of IL-2, IL-6, IFN-γ and TNF-α with the corresponding Lumit® Cytokine Immunoassay. This simple approach for profiling cytokine release can be applied to multiple cell models.

Featured Application Note

CAR T cell-mediated cytotoxicity and cytokine release in response to antigen expression on target cells

Explore a multiplexing approach that unlocks the full potential of CAR T cell therapy with our Lumit® IFN-γ and TNF-α (Human) Immunoassays.

View Application Note

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

Can’t find your target in the list?

Please enquire about early access to new assays in development.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocols

Complete Protocol

Specifications

You are viewing: W6040 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 1 × 160μl

Lumit® Detection Buffer B

 VB406A 1 × 3.2ml

Human IFN-γ Standard

 W134A 1 × 25μl 10μg/ml

Anti-hIFN-γ mAb-SmBiT, 500X

 W135A 1 × 30μl

Anti-hIFN-γ mAb-LgBiT, 500X

 W136A 1 × 30μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6042 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 5 × 160μl

Lumit® Detection Buffer B

 VB406A 5 × 3.2ml

Human IFN-γ Standard

 W134A 1 × 25μl 10μg/ml

Anti-hIFN-γ mAb-SmBiT, 500X

 W135A 5 × 30μl

Anti-hIFN-γ mAb-LgBiT, 500X

 W136A 5 × 30μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6041 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405B 1 × 1.25ml

Lumit® Detection Buffer B

 VB406B 1 × 25ml

Human IFN-γ Standard

 W134A 1 × 25μl 10μg/ml

Anti-hIFN-γ mAb-SmBiT, 500X

 W135B 1 × 300μl

Anti-hIFN-γ mAb-LgBiT, 500X

 W136B 1 × 300μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Resources

Articles

Posters

Other Resources

Similar Products

Lumit® IL-1β Human/Mouse Immunoassay

Quantifies inflammasome activation by measuring released IL-1β using a simple, no-wash protocol.

W6010, W6011, W6012, W7010, W7011, W7012

Lumit® HMGB1 Human/Mouse Immunoassay

HMGB1 quantitation with an add-mix-measure protocol completed in 90 minutes or less.

W6110, W6112

Lumit® Immunoassay Labeling Kit and Detection Reagents

Antibody labeling kit and detection reagents to help develop Lumit® Immunoassays.

VB2500, VB2010, VB2020, VB2030, VB4050, VB4060

Lumit® IL-2 (Human) Immunoassay

Quantitatively measures released IL-2 in cell culture samples using a simple, no-wash protocol.

W6020, W6022, W6021

Lumit® TNF-α (Human) Immunoassay

Quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol.

W6050, W6052, W6051

Frequently Used With

Caspase-Glo® 1 Inflammasome Assay

A homogeneous, bioluminescent method to selectively measure the activity of caspase-1, an essential component of the inflammasome.

G9951, G9952, G9953

LDH-Glo™ Cytotoxicity Assay

Bioluminescence-based LDH assay for sensitive detection of cytotoxicity in samples low in cell number, including 3D microtissues.

J2380, J2381

T Cell Activation Bioassay (NFAT/IL-2)

Choose NFAT or IL-2 kit to assess activity of biologics designed to bind and activate T cells via engagement of TCR/CD3.

J1621, J1625, J1601, J1651, J1655, J1631

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

Let's find the product that meets your needs.

Talk to a Scientist

Sylvia

Silvia

Italy
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.