Lumit® IL-1β Human/Mouse Immunoassay

W6010_Lumit-Human-IL-1beta-Immunoassay_3
W6011_Lumit-Human-IL-1beta-Immunoassay_3
W6012_Lumit-Human-IL-1beta-Immunoassay_3
W6010_Lumit-Human-IL-1beta-Immunoassay_3
automation-support-promo
customize-this-small

No-Wash Immunoassay for IL-1β Detection in Cells

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a standard plate-reading luminometer
  • Scalable to 96- or 384-well plates

Choose an antibody

Size

Catalog number selected: W6010

$ 597.00
Your price:
Add to Cart
This product is discontinued
Request a Sample
Not available in all countries Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Lumit® IL-1β Human/Mouse Immunoassay
Human/100 assays
$ 597.00
Your price: Log in
Change Configuration

Quantify Inflammasome Activation Without Tedious Wash Steps

The Lumit® IL-1β Immunoassay quantitatively measures released IL-1β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® IL-1β Immunoassay Works

Primary antibodies to IL-1β were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-1β, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-1β levels.
16522ma

Simple One-Plate Protocol, No Wash Steps!

16545ma

Large Dynamic Range of Lumit® Detection

The picomolar to nanomolar linear range of Lumit® detection covers expected concentrations for most cell models.

Linear range of lumit IL-1b human immunoassay
Linear range of lumit IL-1b mouse immunoassay

Assay

IL-1β (Human) Immunoassay

IL-1β (Mouse) Immunoassay

Limit of Detection (LOD)

10pg/ml (3 SD above background)

8pg/ml (3 SD above background)

Dynamic Range

22pg/ml – 40ng/ml

11pg/ml – 20ng/ml

Minimal Detectable Dose (MDD)

7pg/ml (2 SD above background)

5pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

High-Throughput NLRP3 Inflammasome Inhibitor Screening

The add-and-read format of the Lumit® IL-1β Immunoassay enables high-throughput screening for modulators of IL-1β release.
IL-1b inhibitor high throughput screening

The NLRP3 inflammasome inhibitor, MCC950, prevents caspase-1 activation and release of mature IL-1β. A titration of MCC950 was added to differentiated THP-1 cells (5 × 104/well) followed by LPS (20 EU/ml) or vehicle. The Lumit® IL-1β (Human) Immunoassay was performed after 5 hours.

Featured Publication

This peer-reviewed study "Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays" examines the direct engagement and functionality of NLRP3 pathway activity in cells using several mechanistic assays, including the Lumit® IL-1β Immunoassay and Caspase-Glo 1 Inflammasome Assay.

nlrp3-martha-obrien-paper

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

Can’t find your target in the list?

Please enquire about early access to new assays in development.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocols

Complete Protocol

Specifications

You are viewing: W6010 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 1 × 160μl

Lumit® Detection Buffer B

 VB406A 1 × 3.2ml

Human IL-1β Standard

 W116A 1 × 25μl 10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

 W117A 1 × 15μl

Anti hIL-1β mAb-LgBiT, 1000X

 W118A 1 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W6011 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405B 1 × 1.25ml

Lumit® Detection Buffer B

 VB406B 1 × 25ml

Human IL-1β Standard

 W116A 1 × 25μl 10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

 W117B 1 × 150μl

Anti hIL-1β mAb-LgBiT, 1000X

 W118B 1 × 150μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W6012 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 5 × 160μl

Lumit® Detection Buffer B

 VB406A 5 × 3.2ml

Human IL-1β Standard

 W116A 1 × 25μl 10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

 W117A 5 × 15μl

Anti hIL-1β mAb-LgBiT, 1000X

 W118A 5 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W7010 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 1 × 160μl

Lumit® Detection Buffer B

 VB406A 1 × 3.2ml

Mouse IL-1β Standard

 W119A 1 × 25μl 10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

 W120A 1 × 15μl

Anti mIL-1β mAb-LgBiT, 1000X

 W121A 1 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W7011 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405B 1 × 1.25ml

Lumit® Detection Buffer B

 VB406B 1 × 25ml

Mouse IL-1β Standard

 W119A 1 × 25μl 10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

 W120B 1 × 150μl

Anti mIL-1β mAb-LgBiT, 1000X

 W121B 1 × 150μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W7012 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 5 × 160μl

Lumit® Detection Buffer B

 VB406A 5 × 3.2ml

Mouse IL-1β Standard

 W119A 1 × 25μl 10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

 W120A 5 × 15μl

Anti mIL-1β mAb-LgBiT, 1000X

 W121A 5 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Resources

Articles

Posters

Other Resources

Similar Products

Lumit® TNF-α (Human) Immunoassay

Quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol.

W6050, W6052, W6051

Lumit® IL-6 (Human) Immunoassay

Quantitatively measures released IL-6 in cell culture samples using a simple, no-wash protocol.

W6030, W6032, W6031

Lumit® Active IL-18 (Human) Immunoassay

Quantitatively measure active IL-18 in cell culture samples using a simple, no-wash protocol completed in 70 minutes or less.

Caspase-Glo® 1 Inflammasome Assay

A homogeneous, bioluminescent method to selectively measure the activity of caspase-1, an essential component of the inflammasome.

G9951, G9952, G9953

Frequently Used With

LDH-Glo™ Cytotoxicity Assay

Bioluminescence-based LDH assay for sensitive detection of cytotoxicity in samples low in cell number, including 3D microtissues.

J2380, J2381

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

Let's find the product that meets your needs.

Talk to a Scientist

Petra

Petra

Germany
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.