RealTime-Glo™ Extracellular ATP Assay

How To Measure Immunogenic Cell Death In Real Time
GA5010_RealTime-Glo-TM--Extracellular-ATP-Assay--200-assays_3
GA5011_RealTime-Glo-TM--Extracellular-ATP-Assay--2000-assays_3
GA5012_RealTime-Glo-TM--Extracellular-ATP-Assay--10-x-200-assays_3
how-to-measure-immunogenic-cell-death-in-real-time-fallback
customize-this-small
automation-support-promo

Live-Cell Kinetic Assay for Measuring Extracellular ATP

  • Get more data per well with real-time monitoring
  • Just add, mix, measure; no supernatant sampling
  • Ideal for high-throughput applications

Size

Catalog number selected: GA5010

$ 230.00
Your price:
Add to Cart
This product is discontinued
Request a Sample
Not available in all countries Savings Calculator Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
RealTime-Glo™ Extracellular ATP Assay
200 assays
$ 230.00
Your price: Log in
Change Configuration

Monitor Immunogenic Cell Death in Real Time

The RealTime-Glo™ Extracellular ATP Assay is a bioluminescent assay designed for kinetic monitoring of ATP released from dying, stressed or activated cells. Extracellular ATP can function as a damage associated molecular pattern (DAMP) and is a key biomarker for determining whether a treatment induces immunogenic cell death, a specialized form of cell death that results in an immune response.

The RealTime-Glo™ Extracellular ATP Assay allows you to continually monitor extracellular ATP so you won’t miss crucial time points. It is compatible for use in 384-well plates, making it ideal for high-throughput screening applications.

Simple Protocol for Measuring Extracellular ATP

Set up one plate to collect all the time points you need! First, reconstitute the reagent with cell culture medium and add to cells at the time of treatment. Then, read the luminescent signal continuously for 24 hours or longer using a standard plate-reading luminometer. No supernatant sampling required.

RealTime-Glo Extracellular ATP Assay Workflow

Kinetic Monitoring of Immunogenic Cell Death

Kinetic Monitoring of Immunogenic Cell Death

U937 cells were treated with a dilution of mitoxantrone, an anthracycline shown to induce immunogenic cell death (ICD). RealTime-Glo™ Extracellular ATP Assay Reagent was added, and the assay plate was placed in a plate reader set at 37°C. Luminescence was collected every 10 minutes for 24 hours.

Compatible with 2D and 3D Cell Models 

Compatible with 2D Cell Models
Compatible with 3D Cell Models

HCT-116 cells were grown in monolayer (left) and in spheroids using ultra-low attachment plates (right). Cells were treated with dilutions of staurosporine, and the RealTime-Glo™ Extracellular ATP Assay Reagent was added. The timing for ATP release differs between monolayer and spheroid cell culture, and the reagent is compatible with both cell culture models.

Want to measure ATP as a marker of cell viability?

Learn about the CellTiter-Glo® 2.0 Assay, a stable, single-reagent ATP assay.

Also Available: Lumit® HMGB1 Human/Mouse Immunoassay

The Lumit® HMGB1 Immunoassay detects HMGB1 directly in cell culture samples in 90 minutes or less and streamlines your DAMP and immunogenic cell death research. Use this assay to complement the RealTime-Glo™ Extracellular ATP Assay for a more complete picture of immunogenic cell death.

Protocols

Complete Protocol

Specifications

You are viewing: GA5010 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

 GA501A 1 × 1 vial

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: GA5011 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

 GA501B 1 × 1 vial

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: GA5012 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

 GA501A 10 × 1 vial

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Articles

Posters

Other Resources

Similar Products

RealTime-Glo™ Apoptosis and Necrosis Assay

A one-step, plate-based assay for continually monitoring apoptosis progression.

JA1011, JA1012, JA1000, JA1001

RealTime-Glo™ MT Cell Viability Assay

A bioluminescent method to kinetically monitor viability in cell culture up to 72 hours.

G9711, G9712, G9713

Lumit® HMGB1 Human/Mouse Immunoassay

HMGB1 quantitation with an add-mix-measure protocol completed in 90 minutes or less.

W6110, W6112

Frequently Used With

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

CellTox™ Green Cytotoxicity Assay

Measures changes in membrane integrity. Kinetically monitors cytotoxicity up to 72 hours with multiplex capability.

G8741, G8742, G8743, G8731

Caspase-Glo® 1 Inflammasome Assay

A homogeneous, bioluminescent method to selectively measure the activity of caspase-1, an essential component of the inflammasome.

G9951, G9952, G9953

Lumit® IL-1β Human/Mouse Immunoassay

Quantifies inflammasome activation by measuring released IL-1β using a simple, no-wash protocol.

W6010, W6011, W6012, W7010, W7011, W7012

Let's find the product that meets your needs.

Talk to a Scientist

Elisa

Elisa

Spain
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.