GSH-Glo™ Glutathione Assay

Sensitively Detect Total and Reduced GSH in Cells

The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. A change in GSH levels is important when assessing toxicological responses and an indicator of oxidative stress, potentially leading to apoptosis or cell death. The GSH detection assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH. The reaction is catalyzed by a glutathione S-transferase (GST) enzyme supplied in the kit. The luciferin formed is detected in a coupled reaction using Ultra-Glo™ Recombinant Luciferase that generates a glow-type luminescence that is proportional to the amount of glutathione present in cells. The GSH detection assay provides a simple, fast and sensitive alternative to colorimetric and fluorescent methods and can be adapted easily to high-throughput applications.