PIK3R1 Expression Vector

The PIK3R1 Expression Vector contains the coding region of the human PIK3R1 regulatory protein. The expression vectors are designed for use with kinase-NanoLuc® Fusion Vectors that contain the coding region of a kinase-NanoLuc® (or NanoLuc®-kinase) fusion protein. The expression vector and kinase-NanoLuc® fusion vector are cotransfected into cells prior to analysis using the appropriate NanoBRET® Target Engagement Intracellular Kinase Assay. These regulatory protein expression vectors are supplied transfection-ready, with a CMV promoter that drives expression of the full-length regulatory protein.

For details on kinase-NanoLuc® Fusion Vectors that require co-expression with a regulatory protein expression vector, see the NanoBRET® Target Engagement Intracellular Kinase Assay Adherent Format Technical Manual, #TM598.

Vector Details:

• Gene name: PIK3R1
• Sequence
• Recommended Assay: NanoBRET® TE Intracellular Kinase Assay, Adherent format