JM109 Competent Cells

L1001_JM109 Competent Cells_3
customize-this-small
View information on global supply logistics

Ready-to-Use Competent E. coli Cells for Recombinant Cloning and Subcloning

  • Cells are ready to use—no preparation time necessary
  • Transformation efficiencies guaranteed
  • No worries about undesirable recombination events (recA– mutation), and no nuclease carryover in miniprep DNA (endA– mutation)

Choose usage

Size

Catalog number selected: L2005

$ 224.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
JM109 Competent Cells
Single Use/1ml
(20 × 50µl)
$ 224.00
Your price: Log in
Change Configuration

JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg for Cloning and Single Use, and Subcloning Efficiency at greater than 107cfu/μg.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

JM109 Competent Cells are prepared according to a modified procedure of Hanahan.

JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].

Reference

  1. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: L2005 Change Configuration

What's in the box?

Item Part # Size

Single-Use JM109 Competent Cells, >108cfu/µg

 L200B 20 × 50μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L1001 Change Configuration

What's in the box?

Item Part # Size

JM109 Competent Cells, Subcloning Efficiency

 L100A 5 × 200μl

Competent Cells Control DNA

 L107A 1 × 30μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2001 Change Configuration

What's in the box?

Item Part # Size

Competent Cells Control DNA

 L107A 1 × 30μl

JM109 Competent Cells, High Efficiency

 L200A 5 × 200μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Resources

Articles

Citations

Similar Products

HB101 Competent Cells

E. coli strain ready for transformation. No blue/white selection.

L2015, L2011

Single Step (KRX) Competent Cells

Ideal E. coli cells for recombinant protein expression, offering efficient transformation and regulated protein expression.

L3002

Frequently Used With

IPTG, Dioxane-Free

Inducer of beta-galactosidase activity in many bacteria.

V3955, V3951, V3953

X-Gal

Detect beta-galactosidase activity to differentiate recombinants from nonrecombinants in cloning experiments.

V3941

Let's find the product that meets your needs.

Talk to a Scientist

Joliene

Joliene

US
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.