JM109 Competent Cells

L1001_JM109 Competent Cells_3
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Ready-to-Use Competent E. coli Cells for Recombinant Cloning and Subcloning

  • Cells are ready to use—no preparation time necessary
  • Transformation efficiencies guaranteed
  • No worries about undesirable recombination events (recA– mutation), and no nuclease carryover in miniprep DNA (endA– mutation)

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Catalog number selected: L2005

$ 224.00
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This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

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This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
JM109 Competent Cells
Single Use/1ml
(20 × 50µl)
$ 224.00
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JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg for Cloning and Single Use, and Subcloning Efficiency at greater than 107cfu/μg.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

JM109 Competent Cells are prepared according to a modified procedure of Hanahan.

JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].

Reference

  1. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: L2005 Change Configuration

What's in the box?

Item Part # Size

Single-Use JM109 Competent Cells, >108cfu/µg

 L200B 20 × 50μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L1001 Change Configuration

What's in the box?

Item Part # Size

JM109 Competent Cells, Subcloning Efficiency

 L100A 5 × 200μl

Competent Cells Control DNA

 L107A 1 × 30μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2001 Change Configuration

What's in the box?

Item Part # Size

Competent Cells Control DNA

 L107A 1 × 30μl

JM109 Competent Cells, High Efficiency

 L200A 5 × 200μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Resources

Articles

Citations

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L3002

Frequently Used With

IPTG, Dioxane-Free

Inducer of beta-galactosidase activity in many bacteria.

V3955, V3951, V3953

X-Gal

Detect beta-galactosidase activity to differentiate recombinants from nonrecombinants in cloning experiments.

V3941

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