DNA Step Ladders have defined sizes with exact incremental steps between bands. The ladders are not intended for use in quantitative analysis. The DNA fragments may be stained with ethidium bromide.
The 100bp DNA Step Ladder has 40 blunt-ended DNA fragments ranging from 100bp to 4,000bp in 100bp increments. Two internal features facilitate band identification. A high-intensity 500bp band stands out at the lowest segment of the ladder (<1kb). Bands within each segment (<1kb, <2kb, <4kb) have approximately the same intensity.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 1mM EDTA.