RQ1 RNase-Free DNase

RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO₄, 10mM CaCl₂) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C])

Applications

• Preparation of DNA-free RNA.
• Degradation of DNA from RNA transcription systems.
• Nick translation of DNA.
• Studying DNA:protein interactions by DNase I footprinting.

Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl₂, 10mM MgCl₂.

Source: Bovine pancreas.

QC Tests: Activity, RNase.

Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl₂, 10mM CaCl₂.