S1 Nuclease

S1 Nuclease is an endonuclease that degrades ssDNA and RNA. The enzyme is used to remove protruding single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts. S1 Nuclease is provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO₄.

References

1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192–200.
2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760–4.
3. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 1274–8.

Storage Buffer: 20mM Tris-HCl (pH 7.5 at 25°C), 0.1mM ZnCl₂, 50mM NaCl and 50% (v/v) glycerol.

Source: Fungal α amylase powder.

QC Tests: Activity, unidirectional deletions using the Erase-a-Base™ System.

Unit Definition: One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble material per minute at 37°C in 30mM sodium acetate (pH 4.6 at 25°C), 50mM NaCl, 1mM ZnCl₂, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.