AluI

Cut Site:

AG^▼CT
TC_▲GA

Note: For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

Incubation Conditions: Buffer B. 37°C.

Source: Arthrobacter luteus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 100mM sodium citrate, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
75–100%	100%	75–100%	10–25%	10–25%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
143	158	24	16	27	17

Protocols

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
Buffer B 10X Buffer	R002A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
AluI	R628A	1 × 500u

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Other Resources

How to Use Restriction Enzymes: A Resource Guide

Frequently Used With

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

Agarose, LE, Analytical Grade

Reagent for electrophoretic separation of nucleic acids.

V3121, V3125

Ethidium Bromide Solution, Molecular Grade

Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.

H5041

λ DNA/EcoRI + HindIII Markers

Thirteen fragments of 125–21,226bp generated from Lambda DNA digested with EcoRI and HindIII.

G1731