ApaI

Cut Site:

G GGCC^▼C
C_▲CCGG G

Note: Sensitive to overlapping dcm methylation.

Incubation Conditions: Buffer A. 37°C.

Source: Acetobacter pasteurianus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
100%	50–75%	50–75%	<10%	75–100%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
1	12	0	0	0	0

Protocols

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
Buffer A 10X Buffer	R001A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396E	1 × 750μl	10mg/ml	View Product
ApaI	R636A	1 × 5,000u
MULTI-CORE™ 10X Buffer	R999A	1 × 0.25ml		View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Other Resources

How to Use Restriction Enzymes: A Resource Guide

Frequently Used With

LigaFast™ Rapid DNA Ligation System

Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.

M8221, M8225

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

Agarose, LE, Analytical Grade

Reagent for electrophoretic separation of nucleic acids.

V3121, V3125

Ethidium Bromide Solution, Molecular Grade

Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.

H5041

λ DNA/EcoRI + HindIII Markers

Thirteen fragments of 125–21,226bp generated from Lambda DNA digested with EcoRI and HindIII.

G1731