Need a fit-for-purpose lyophilized PCR amplification assay? Learn more

BamHI

R6021_BamHI--2-500u_3
View information on global supply logistics
customize-this-small

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications

Choose a concentration

Size

Catalog number selected: R6021

$ 58.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
BamHI
10u/µl/2,500u
$ 58.00
Your price: Log in
Change Configuration

Cut Site:

G▼GATC  C
C   CTAG▲G

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Usage Notes:

  1. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn2+.
  2. Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.

Incubation Conditions: Buffer E. 37°C.

Source: Bacillus amyloliquefaciens H.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D E MULTI-CORE™
75–100%* 75–100% 75–100% 50–75% 100% 75–100%

*Buffer A is not recommended due to potential star activity.

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
5 3 0 1 1 1

Protocols

Complete Protocol

Specifications

You are viewing: R6021 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Buffer E 10X Buffer

 R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

 R396D 1 × 150μl 10mg/ml View Product

BamHI

 R602A 1 × 2,500u

MULTI-CORE™ 10X Buffer

 R999A 1 × 0.25ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: R6025 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Buffer E 10X Buffer

 R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

 R396E 1 × 750μl 10mg/ml View Product

BamHI

 R602B 1 × 12,500u

MULTI-CORE™ 10X Buffer

 R999A 1 × 0.25ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Citations

Other Resources

Similar Products

BclI

Restriction enzyme that cuts DNA at 50°C and leaves a 5´ overhang.

R6651

Frequently Used With

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

LigaFast™ Rapid DNA Ligation System

Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.

M8221, M8225

Let's find the product that meets your needs.

Talk to a Scientist

Paraj

Paraj

US
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.